钙离子的存在对于登革病毒诱导的细胞毒性因子的细胞毒性活性是必不可少的。
Presence of Ca2+ is obligatory for the cytotoxic activity of dengue virus-induced cytotoxic factor.
作者信息
Khanna M, Chaturvedi U C, Dhawan R, Tekwani B L, Pandey V C
机构信息
Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
出版信息
Immunology. 1991 Jan;72(1):73-8.
The present study was undertaken to investigate the role of calcium ions (Ca2+) in the cytotoxic activity of the cytotoxic factor (CF) produced by T lymphocytes of the dengue type 2 virus (DV)-infected mouse spleen. It was observed that CF prepared in Ca2(+)-free medium had no cytotoxic activity on normal mouse spleen cells suspended in Ca2(+)-free medium but had activity on cells suspended in medium having Ca2+. The cytotoxic activity of CF was restored by substitution with calcium chloride, the optimal dose being 10(-7) M. CF induced influx of Ca2+ as measured by uptake of radiolabelled calcium chloride (45Ca), in the susceptible target cells, macrophages (M phi) and T lymphocytes, but had no effect on CF-resistant B lymphocytes. Calcium channel blocking drugs, like verapamil, nifedipine and diltiazem, inhibited the cytotoxic activity of CF and also the CF-induced influx of 45Ca in M phi and T cells. Thus, presence of Ca2+ is obligatory for the cytotoxic activity of CF and the cell death is associated with increased intracellular Ca2+.
本研究旨在探讨钙离子（Ca2+）在登革2型病毒（DV）感染小鼠脾脏的T淋巴细胞产生的细胞毒性因子（CF）的细胞毒性活性中的作用。观察到在无Ca2+培养基中制备的CF对悬浮于无Ca2+培养基中的正常小鼠脾脏细胞无细胞毒性活性，但对悬浮于含Ca2+培养基中的细胞有活性。用氯化钙替代可恢复CF的细胞毒性活性，最佳剂量为10（-7）M。通过放射性标记氯化钙（45Ca）摄取测定，CF诱导易感靶细胞巨噬细胞（M phi）和T淋巴细胞中Ca2+内流，但对CF抗性B淋巴细胞无影响。钙通道阻滞剂，如维拉帕米、硝苯地平和地尔硫卓，抑制CF的细胞毒性活性以及CF诱导的M phi和T细胞中45Ca内流。因此，Ca2+的存在是CF细胞毒性活性所必需的，细胞死亡与细胞内Ca2+增加有关。
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