登革病毒感染期间巨噬细胞的功能：B细胞的抗原刺激
Macrophage functions during dengue virus infection: antigenic stimulation of B cells.
作者信息
Rizvi N, Chaturvedi U C, Nagar R, Mathur A
机构信息
Department of Microbiology, K.G. Medical College, Lucknow, India.
出版信息
Immunology. 1987 Nov;62(3):493-8.
This study was undertaken to investigate the function of dengue type 2 virus (DV)-infected mouse peritoneal macrophages (M phi) regarding the antigenic stimulation of B lymphocytes of the spleen. It was observed that a variable proportion of M luminal diameter show DV-specific immunofluorescent antigen, which depended upon the route of administration of the virus, being higher in i.p.-inoculated mice and in vitro-infected M luminal diameter monolayers. The DV-infected M luminal diameter presented the DV antigen to B cells in vitro and in vivo, leading to their clonal expansion as shown by counting the virus-specific IgM antibody plaque-forming cells (PFC). The PFC response depended upon the number of DV-infected M luminal diameter. The antigen was presented equally well both by I-A-negative and I-A-positive M luminal diameter. Superimposition of a heterologous antigen (Coxsackie B4 virus) in a Mackaness type of experiment depressed the capacity of M luminal diameter to present both the homologous as well as heterologous antigen.
本研究旨在探讨登革2型病毒（DV）感染的小鼠腹腔巨噬细胞（M phi）在脾B淋巴细胞抗原刺激方面的功能。观察到不同比例的M管腔直径呈现DV特异性免疫荧光抗原，这取决于病毒的给药途径，腹腔接种小鼠和体外感染的M管腔直径单层细胞中的比例更高。DV感染的M管腔直径在体外和体内都将DV抗原呈递给B细胞，通过计数病毒特异性IgM抗体空斑形成细胞（PFC）表明这导致了它们的克隆扩增。PFC反应取决于DV感染的M管腔直径的数量。I-A阴性和I-A阳性的M管腔直径呈递抗原的效果相同。在Mackaness类型的实验中叠加异源抗原（柯萨奇B4病毒）会降低M管腔直径呈递同源和异源抗原的能力。
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