Uchimura M, Kenjo T, Kuroshima Y, Fujii A, Yamauchi S
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa.
Nihon Sanka Fujinka Gakkai Zasshi. 1991 Jan;43(1):73-9.
We examined the localization of squamous cell cancer associated antigens (SCCAA) in dysplasia, cancer in situ (CIS) and microinvasive SCC of the uterine cervix, since detection of SCCAA in these subjects is highly effective for early diagnosis. Anti-squamous cell cancer associated antibody (Anti SCCAAb IgG) was prepared by immunizing rabbits with specific components at around PI 6.1 that were originally purified from SCC of maxillary sinus. In this study, the following results were obtained by the immunoperoxidase method. (1) Eleven out of 15(73%) cases of dysplasia, 20 out of 26(77%) cases of CIS, 21 out of 24(88%) cases of Stage I, 13 out of 14(93%) cases of Stage II and 9 out of 10(90%) cases of Stage III-IV in the clinical stages of SCC showed positive staining, while controls of unrelated SCC were almost negative. (2) The SCCAA positive ratio was 2 out of 2 cases of small cell nonkeratinized type, 86% in large cell nonkeratinized type and 94% in Keratinized type of SCC. (3) The SCCAA was demonstrated on all the layers of stratified squamous epithelium in a lesion of CIS and some layers migrated to adjacent nonneoplastic lesion with lateral invasion in middle layer. These results suggest that the demonstration of SCCAA may be useful in diagnosing the malignant transformation of squamous epithelium in the early stage of SCC.
我们检测了鳞状细胞癌相关抗原（SCCAA）在子宫颈发育异常、原位癌（CIS）和微浸润性鳞状细胞癌中的定位，因为在这些病变中检测SCCAA对早期诊断非常有效。抗鳞状细胞癌相关抗体（抗SCCAAb IgG）是通过用最初从上颌窦鳞状细胞癌中纯化的、等电点在6.1左右的特定成分免疫兔子制备的。在本研究中，通过免疫过氧化物酶法获得了以下结果。（1）在鳞状细胞癌临床分期中，15例发育异常中有11例（73%）、26例CIS中有20例（77%）、24例I期中有21例（88%）、14例II期中有13例（93%）以及10例III-IV期中有9例（90%）呈阳性染色，而无关鳞状细胞癌对照几乎均为阴性。（2）小细胞非角化型鳞状细胞癌2例中有2例SCCAA阳性率为100%，大细胞非角化型为86%，角化型为94%。（3）在CIS病变的复层鳞状上皮各层均显示有SCCAA，在中层有侧向浸润时，部分SCCAA迁移至邻近的非肿瘤性病变。这些结果表明，SCCAA的显示可能有助于鳞状细胞癌早期阶段鳞状上皮恶性转化的诊断。
