Olsen R A, Seewald M J, Melder D C, Berggren M, Iaizzo P A, Powis G
Department of Pharmacology, Mayo Clinic, Rochester, MN.
Toxicol Lett. 1991 Jan;55(1):117-25. doi: 10.1016/0378-4274(91)90033-3.
Platelet-derived growth factor (PDGF) produced an almost complete block of the increase in intracellular free Ca2+ concentration ([Ca2+]i) in Swiss 3T3 fibroblasts caused by the Ca2(+)-selective ionophores 4-bromo-A23187 and ionomycin, and by the volatile anesthetic agent halothane. The effect of PDGF was similar to the decreased [Ca2+]i response to Ca2(+)-ionophores produced by phorbol 12-myristate 13-acetate, an activator of protein kinase C. There was no effect of PDGF or PMA on the acute or delayed toxicity of the Ca2(+)-ionophores to Swiss 3T3 cells, suggesting that the increase in [Ca2+]i is not the direct cause of toxicity of these agents.
血小板衍生生长因子（PDGF）几乎完全阻断了由钙离子选择性离子载体4-溴-A23187和离子霉素以及挥发性麻醉剂氟烷引起的瑞士3T3成纤维细胞内游离钙离子浓度（[Ca2+]i）的升高。PDGF的作用类似于佛波酯12-肉豆蔻酸酯13-乙酸酯（一种蛋白激酶C激活剂）所产生的对钙离子载体引起的[Ca2+]i反应降低。PDGF或佛波酯对钙离子载体对瑞士3T3细胞的急性或延迟毒性均无影响，这表明[Ca2+]i的升高并非这些药物毒性的直接原因。
