Tsuda T, Kaibuchi K, West B, Takai Y
FEBS Lett. 1985 Jul 22;187(1):43-6. doi: 10.1016/0014-5793(85)81210-2.
Addition of platelet-derived growth factor (PDGF) to quiescent cultures of Swiss 3T3 fibroblasts rapidly increased the cytoplasmic free Ca2+ concentrations and afterwards the c-myc mRNA levels. The concentrations of PDGF needed for both reactions were roughly the same. Moreover, the Ca2+ ionophores, A23187 and ionomycin, mimicked the actions of PDGF and increased the c-myc mRNA levels. These results suggest that Ca2+ may be involved in the regulation of PDGF-induced expression of the c-myc gene.
向瑞士3T3成纤维细胞的静止培养物中添加血小板衍生生长因子（PDGF），可迅速提高细胞质游离Ca2+浓度，随后提高c-myc mRNA水平。这两种反应所需的PDGF浓度大致相同。此外，Ca2+离子载体A23187和离子霉素模拟了PDGF的作用，提高了c-myc mRNA水平。这些结果表明，Ca2+可能参与了PDGF诱导的c-myc基因表达的调控。
