Le Huërou I, Guilloteau P, Toullec R, Puigserver A, Wicker C
Centre de Biochemie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
Biochem Biophys Res Commun. 1991 Feb 28;175(1):110-6. doi: 10.1016/s0006-291x(05)81207-0.
A full-length cDNA clone encoding bovine pancreatic preprocarboxypeptidase A was isolated and sequenced. The 1405-base pair insert contains a 26-nucleotide 5'-noncoding region, a 1260-nucleotide open reading frame and a 76-nucleotide 3'-noncoding fragment plus a poly(A) tail of at least 43 nucleotides. The open reading frame encodes a protein of 419 amino acids, including the 16 amino acid signal peptide. The mature enzyme (309 residues) has two additional C-terminal amino acids, as compared with the amino acid sequence of the protein which was reported more than 20 years ago. In addition, four residues deduced from the nucleotide sequence differed from those identified in the reported amino acid sequence from their net charge: Asp-89, Asp-114, Gln-122, and Asp-185 instead of Asn-89, Asn-114, Glu-122, and Asn-185, respectively. A high degree of identity exists between the nucleotide sequences (81.3%), on the one hand, and the amino acid sequences (78.3%), on the other hand, of bovine preprocarboxypeptidase A and rat preprocarboxypeptidase A1.
分离并测序了编码牛胰前羧肽酶A的全长cDNA克隆。1405个碱基对的插入片段包含一个26个核苷酸的5'-非编码区、一个1260个核苷酸的开放阅读框和一个76个核苷酸的3'-非编码片段以及至少43个核苷酸的聚腺苷酸尾巴。该开放阅读框编码一个由419个氨基酸组成的蛋白质，包括16个氨基酸的信号肽。与20多年前报道的蛋白质氨基酸序列相比，成熟酶（309个残基）有另外两个C末端氨基酸。此外，从核苷酸序列推导的四个残基与其在报道的氨基酸序列中确定的残基在净电荷方面存在差异：分别为Asp-89、Asp-114、Gln-122和Asp-185，而不是Asn-89、Asn-114、Glu-122和Asn-185。牛前羧肽酶A和大鼠前羧肽酶A1的核苷酸序列（81.3%）以及氨基酸序列（78.3%）之间存在高度同一性。
