Le Huerou I, Wicker C, Guilloteau P, Toullec R, Puigserver A
Centre de Biochimie et de Biologie Moléculaire du Centre National de la Recherche Scientifique, Marseille, France.
Eur J Biochem. 1990 Nov 13;193(3):767-73. doi: 10.1111/j.1432-1033.1990.tb19398.x.
A cDNA clone encoding an anionic form of bovine trypsinogen was isolated from a pancreatic cDNA library. The corresponding 855-nucleotide mRNA contains a short 5' noncoding region of 8 nucleotides and a long 3' noncoding region of 56 nucleotides in addition to a poly(A) tail of at least 50 nucleotides. The deduced amino acid sequence for the anionic pretrypsinogen (247 residues) includes the N-terminal 15-amino-acid signal peptide followed by an 8-amino-acid activation peptide. The zymogen (232 residues) contains an additional C-terminal serine, compared with the amino acid sequence of bovine cationic trypsinogen. The identity between the anionic and cationic forms of bovine trypsinogen (65%) is lower than that existing between the anionic protein and other mammalian anionic trypsinogens (73-85%), suggesting that trypsin gene duplication in mammals occurred prior to the evolutionary events responsible for the species divergence. Bovine pancreatic anionic trypsin possesses all the key amino acids characteristic of the serine protease family.
从牛胰腺cDNA文库中分离出一个编码阴离子型牛胰蛋白酶原的cDNA克隆。相应的855个核苷酸的mRNA除了有至少50个核苷酸的聚腺苷酸尾巴外，还包含一个8个核苷酸的短5'非编码区和一个56个核苷酸的长3'非编码区。推导的阴离子型前胰蛋白酶原（247个残基）的氨基酸序列包括N端的15个氨基酸信号肽，其后是一个8个氨基酸的激活肽。与牛阳离子型胰蛋白酶原的氨基酸序列相比，该酶原（232个残基）在C端还含有一个丝氨酸。牛胰蛋白酶原阴离子型和阳离子型之间的同一性（65%）低于阴离子型蛋白质与其他哺乳动物阴离子型胰蛋白酶原之间的同一性（73-85%），这表明哺乳动物中的胰蛋白酶基因复制发生在导致物种分化的进化事件之前。牛胰腺阴离子型胰蛋白酶具有丝氨酸蛋白酶家族所有关键的氨基酸特征。
