zu Putlitz J, Datta S, Madison L D, Macchia E, Jameson J L
Thyroid Unit, Massachusetts General Hospital, Boston.
Biochem Biophys Res Commun. 1991 Feb 28;175(1):285-90. doi: 10.1016/s0006-291x(05)81232-x.
Thyroid hormone receptors are nuclear proteins which regulate transcription in a hormone dependent manner. The baculovirus expression system was used for the overexpression of the beta 1 isoform of the human thyroid hormone receptor. The baculovirus produced receptor binds tri-iodothyronine with high affinity, is specifically immunoprecipitated with a beta 1 specific antibody, and binds to DNA that contains a known thyroid hormone receptor recognition site. Large scale production and purification of baculovirus produced receptor will be useful for structure-function analyses and studies of transcriptional regulation.
甲状腺激素受体是一种核蛋白，它以激素依赖的方式调节转录。杆状病毒表达系统用于人甲状腺激素受体β1亚型的过表达。杆状病毒产生的受体以高亲和力结合三碘甲状腺原氨酸，可被β1特异性抗体特异性免疫沉淀，并与含有已知甲状腺激素受体识别位点的DNA结合。大规模生产和纯化杆状病毒产生的受体将有助于进行结构功能分析和转录调控研究。
