Hallenbeck P L, Marks M S, Lippoldt R E, Ozato K, Nikodem V M
Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5572-6. doi: 10.1073/pnas.89.12.5572.
Steroid/TH receptors mediate transcriptional induction of promoters containing hormone response elements (HREs) through an unclear mechanism that involves receptor binding to both hormone and a HRE. Here we demonstrate that both HRE binding and the transcriptional inducing activities of one member of this family, TH receptor, were markedly enhanced by heterodimerization with H-2RIIBP, a non-TH-binding member of the steroid hormone receptor superfamily. H-2RIIBP, the mouse homologue of human retinoic acid-related receptor, was shown to form stable heterodimers with the TH receptor either in solution or when bound to a TH response element. The results presented indicate that it might be necessary for the TH receptor or other members of this superfamily to have specific partners for heterodimer formation to elicit maximal hormone-specific gene regulation from particular HREs.
类固醇/甲状腺激素受体通过一种不明机制介导含有激素反应元件（HREs）的启动子的转录诱导，该机制涉及受体与激素及HRE的结合。在此我们证明，该家族的一个成员——甲状腺激素受体的HRE结合及转录诱导活性，通过与H - 2RIIBP（类固醇激素受体超家族中一个不结合甲状腺激素的成员）异源二聚化而显著增强。H - 2RIIBP是人类视黄酸相关受体的小鼠同源物，已证实在溶液中或与甲状腺激素反应元件结合时，它能与甲状腺激素受体形成稳定的异源二聚体。所呈现的结果表明，甲状腺激素受体或该超家族的其他成员可能需要有特定的伙伴来形成异源二聚体，以便从特定的HREs引发最大程度的激素特异性基因调控。
