人类嗜T淋巴细胞病毒I型（HTLV-I）蛋白p27rex的核仁定位信号对于p27rex稳定白细胞介素-2受体α亚基mRNA至关重要。
The nucleolar localisation signal of the HTLV-I protein p27rex is important for stabilisation of IL-2 receptor alpha subunit mRNA by p27rex.
作者信息
White K N, Nosaka T, Kanamori H, Hatanaka M, Honjo T
机构信息
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
出版信息
Biochem Biophys Res Commun. 1991 Feb 28;175(1):98-103. doi: 10.1016/s0006-291x(05)81205-7.
In this study we investigated the mechanism of stabilisation of IL-2 receptor alpha subunit mRNA by the HTLV-I protein p27rex. We tested the role of the nucleolar targetting signal in rex by introducing mutations. Three deletion mutants could not express rex protein in the nucleolus and although protein was still expressed in the nucleoplasm none of the mutants could stabilise IL-2R alpha mRNA. A substitution mutant could be expressed in the nucleolus and could also stabilise IL-2R alpha mRNA. The data show that the nucleolar targetting signal is crucial for stabilisation of IL-2R alpha mRNA by rex and raise the possibility that transport of mRNA from nucleus to cytoplasm can involve the nucleolus.
在本研究中，我们调查了人嗜T淋巴细胞病毒I型（HTLV-I）蛋白p27rex对白细胞介素-2受体α亚基（IL-2受体α亚基）信使核糖核酸（mRNA）的稳定机制。我们通过引入突变来测试核仁靶向信号在rex中的作用。三个缺失突变体无法在核仁中表达rex蛋白，并且尽管蛋白仍在核质中表达，但没有一个突变体能稳定IL-2Rα mRNA。一个替代突变体可以在核仁中表达，并且也能稳定IL-2Rα mRNA。数据表明，核仁靶向信号对于rex稳定IL-2Rα mRNA至关重要，并增加了mRNA从细胞核运输到细胞质可能涉及核仁的可能性。
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