在存在或不存在[K⁺, Cl⁻]共转运系统的特异性抑制剂DIOA的情况下，脱氧镰状细胞中的K⁺外流。

K+ efflux in deoxygenated sickle cells in the presence or absence of DIOA, a specific inhibitor of the [K+, Cl-] cotransport system.

作者信息

Olivieri O, Vitoux D, Bachir D, Beuzard Y

机构信息

INSERM U91, Hôpital Henri Mondor, Créteil, France.

出版信息

Br J Haematol. 1991 Jan;77(1):117-20. doi: 10.1111/j.1365-2141.1991.tb07957.x.

DOI:10.1111/j.1365-2141.1991.tb07957.x

PMID:1998586

Abstract

The ouabain bumetanide resistant (OBR) K+ efflux was investigated in deoxygenated sickle cells in comparison to oxygenated ones, by using a specific inhibitor of the [K+, Cl-] co-transport system, [(DihydroIndenyl)Oxy] Alkanoic acid (DIOA). A DIOA sensitive and a DIOA resistant K+ efflux were measured in deoxygenated sickle cells. The DIOA sensitive K+ efflux shared the properties of the [K+, Cl-] co-transport system, being stimulated by decreased pH and hypoosmolarity. This DIOA sensitive K+ efflux represented 70% of the total K+ efflux at pH 7.0 and at low pO2 (10-15 mmHg). Thus, a small reduction in Ph effectively stimulated the [K+, Cl-] co-transport system in deoxygenated condition, and this may contribute significantly to the sickle cell dehydration. We conclude that at pH lower than 7.4, the [K+, Cl-] co-transport system is permanently activated in sickle cells and leads to sickle cell dehydration in both oxygenated and deoxygenated conditions.

摘要

通过使用[K⁺, Cl⁻]共转运系统的特异性抑制剂[(二氢茚基)氧基]链烷酸（DIOA），研究了脱氧镰状细胞与氧合镰状细胞相比的哇巴因布美他尼抗性（OBR）钾外流情况。在脱氧镰状细胞中测量了对DIOA敏感和对DIOA抗性的钾外流。对DIOA敏感的钾外流具有[K⁺, Cl⁻]共转运系统的特性，受pH降低和低渗刺激。在pH 7.0和低氧分压（10 - 15 mmHg）时，这种对DIOA敏感的钾外流占总钾外流的70%。因此，pH的小幅降低在脱氧条件下有效刺激了[K⁺, Cl⁻]共转运系统，这可能对镰状细胞脱水有显著贡献。我们得出结论，在pH低于7.4时，[K⁺, Cl⁻]共转运系统在镰状细胞中被永久激活，并在氧合和脱氧条件下导致镰状细胞脱水。