Stein O, Dabach Y, Hollander G, Stein Y
Department of Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Biochim Biophys Acta. 1991 Jan 28;1081(2):238-40. doi: 10.1016/0005-2760(91)90031-c.
The putative role of cholesteryl ester transfer protein (CETP) in the removal of cholesteryl ester from hepatic reticuloendothelial cells in vivo was studied in hamsters. The parameter tested was retention of [3H]cholesteryl linoleyl ether ([3H]CLE), a nonhydrolysable analog of cholesteryl ester, in the liver after injection of [3H]CLE labeled acetylated LDL, which is targetted to nonparenchymatous littoral cells. In hamsters fed laboratory chow, plasma cholesteryl ester transfer activity (CETA) was 10.6 +/- 0.9 units and the retention of [3H]CLE in the liver 28 days after injection was 86% of the 4 h value. It was about 55% in rats fed the same diet, in which CETA was not detectable. When the diet was supplemented with 2% cholesterol and 15% margarine, CETA activity in hamsters increased 2-fold, yet no change in retention of [3H]CLE in liver was seen after 28 days. In rats, the retention of [3H]CLE in the liver was also not changed by the dietary fat supplementation. These results do not support the role of CETP in vivo in removal of cholesteryl ester from intact reticuloendothelial cells.
在仓鼠体内研究了胆固醇酯转运蛋白（CETP）在从肝网状内皮细胞清除胆固醇酯中的假定作用。所测试的参数是注射靶向非实质边缘细胞的[3H]胆固醇亚油酸醚（[3H]CLE，一种胆固醇酯的不可水解类似物）标记的乙酰化低密度脂蛋白后，肝脏中[3H]CLE的滞留情况。在喂食实验室饲料的仓鼠中，血浆胆固醇酯转移活性（CETA）为10.6±0.9单位，注射后28天肝脏中[3H]CLE的滞留量是4小时值的86%。在喂食相同饲料的大鼠中约为55%，在大鼠中未检测到CETA。当饲料补充2%胆固醇和15%人造黄油时，仓鼠的CETA活性增加了2倍，但28天后肝脏中[3H]CLE的滞留量未见变化。在大鼠中，膳食脂肪补充也未改变肝脏中[3H]CLE的滞留量。这些结果不支持CETP在体内从完整的网状内皮细胞清除胆固醇酯中的作用。
