Rew D A, Wilson G D, Taylor I, Weaver P C
University Surgical Unit, Southampton General Hospital, UK.
Br J Surg. 1991 Jan;78(1):60-6. doi: 10.1002/bjs.1800780120.
The cell proliferation kinetics of 100 human colonic and rectal adenocarcinomas have been studied in vivo by bromodeoxyuridine infusion and multiparameter flow cytometry. A total of 97 patients, three with synchronous tumours, consented to receive a single bolus dose of 250 mg between 2.4 and 16 h before curative or palliative surgery. By this method, the ploidy pattern, the total and aneuploid labelling indices (LI), the S phase duration (Ts) and the potential doubling time (Tpot) can be estimated. Of the tumours 48 were diploid and 52 were aneuploid. The mean and median total LI of 100 tumours were 9.0 per cent (range 0.7-22.2 per cent). The mean aneuploid LI was 12.1 per cent (median 12.0 per cent, range 2.0-25.5 per cent), and was significantly higher than the total LI (P = 0.01). The labelling index alone is not a sufficient indicator of proliferation, because the Ts also varies within and between tumours. The intertumour range of the Ts varied from 4.0 to 28.6 h. The mean was 14.1 h and the median was 13.1 h. The mean Tpot was 5.9 days (median 3.9 days) with a range of 1.7-21.4 days. No correlation was found between any kinetic parameters and the Dukes' classification or histological classification. The correlation between proliferation and prognosis will be established in due course.
通过溴脱氧尿苷输注和多参数流式细胞术对100例人类结肠直肠癌的细胞增殖动力学进行了体内研究。共有97例患者（3例为同时性肿瘤）同意在根治性或姑息性手术前2.4至16小时接受250毫克的单次推注剂量。通过这种方法，可以估计倍体模式、总标记指数和非整倍体标记指数（LI）、S期持续时间（Ts）和潜在倍增时间（Tpot）。其中48例肿瘤为二倍体，52例为非整倍体。100例肿瘤的平均总LI和中位数总LI分别为9.0%（范围0.7 - 22.2%）。平均非整倍体LI为12.1%（中位数12.0%，范围2.0 - 25.5%），显著高于总LI（P = 0.01）。仅标记指数不足以作为增殖的指标，因为Ts在肿瘤内部和之间也存在差异。Ts的肿瘤间范围为4.0至28.6小时。平均值为14.1小时，中位数为13.1小时。平均Tpot为5.9天（中位数3.9天），范围为1.7至21.4天。未发现任何动力学参数与Dukes分期或组织学分类之间存在相关性。增殖与预后之间的相关性将在适当的时候确定。
