Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India.
J Microbiol Biotechnol. 2009 Nov;19(11):1385-92. doi: 10.4014/jmb.0901.0035.
This work evaluates the effect of different amino acids on production of CyA production in solid-state fermentation that was previously optimized for different fermentation parameters by one-factor-at-a-time for the maximum production of CyA by Tolypocladium inflatum MTCC 557. Based on the Plackett-Burman design, glycerol, ammonium sulfate, FeCl3, and inoculum size were selected for further optimization by response surface methodology (RSM). After identifying effective nutrients, RSM was used to develop mathematical model equations, study responses, and establish the optimum concentrations of the key nutrients for higher CyA production. It was observed that supplementation of medium containing (% w/w) glycerol, 1.53; ammonium sulfate, 0.95; FeCl3, 0.18; and inoculum size 6.4 ml/5g yielded a maximum of 7,106 mg/kg as compared with 6,480 mg CyA/kg substrate using one factor at a time. In the second step, the effect of amino acids on the production of CyA was studied. Addition of L-valine and L-leucine in combination after 20 h of fermentation resulted in maximum production of 8,166 mg/kg.
本研究评估了不同氨基酸对固态发酵中 CyA 产量的影响。先前已通过单因素法对不同发酵参数进行了优化，以获得最大的蛹虫草 MTCC 557 产 CyA。基于 Plackett-Burman 设计，选择甘油、硫酸铵、FeCl3 和接种量进行响应面法（RSM）进一步优化。在确定有效营养物后，RSM 用于开发数学模型方程、研究响应，并确定关键营养物的最佳浓度以实现更高的 CyA 产量。结果表明，与一次一因素法获得的 6480mg/kg 相比，在培养基中添加（w/w）甘油 1.53%、硫酸铵 0.95%、FeCl3 0.18%和接种量 6.4ml/5g 可获得最高 7106mg/kg 的 CyA。在第二步中，研究了氨基酸对 CyA 产量的影响。在发酵 20 小时后添加 L-缬氨酸和 L-亮氨酸的组合可获得最高的 8166mg/kg 的产量。
Zotero 插件