Survase Shrikant A, Shaligram Nikhil S, Pansuriya Ruchir C, Annapure Uday S, Singhal Rekha S
Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India.
J Microbiol Biotechnol. 2009 May;19(5):462-7. doi: 10.4014/jmb.0805.324.
Cyclosporin A (CyA) produced by Tolypocladium inflatum is a promising drug owing to its immunosuppressive and antifungal activities. From an industrial point of view, the necessity to obtain a suitable and economic medium for higher production of CyA was the aim of this work. The present study evaluated the effect of different fermentation parameters in solid state fermentation, such as selection of solid substrate, hydrolysis of substrates, initial moisture content, supplementation of salts, additional carbon, and nitrogen sources, as well as the inoculum age and size, on production of CyA by Tolypocladium inflatum MTCC 557. The fermentation was carried out at 25+/-2 degrees for 9 days. A combination of hydrolyzed wheat bran flour and coconut oil cake (1:1) at 70% initial moisture content supported a maximum production of 3,872+/-156 mg CyA/kg substrate as compared with 792+/-33 mg/kg substrate before optimization. Furthermore, supplementation of salts, glycerol (1%w/w), and ammonium sulfate (1%w/w) increased the production of CyA to 5,454+75 mg/kg substrate. Inoculation of 5 g of solid substrate with 6 ml of 72-h-old seed culture resulted in a maximum production of 6,480+95 mg CyA/kg substrate.
由Inflatum顶头孢霉产生的环孢菌素A（CyA）因其免疫抑制和抗真菌活性而成为一种有前景的药物。从工业角度来看，获得适合且经济的培养基以提高CyA产量是这项工作的目标。本研究评估了固态发酵中不同发酵参数的影响，例如固体底物的选择、底物的水解、初始水分含量、盐的添加、额外的碳源和氮源，以及接种物的菌龄和大小，对Inflatum顶头孢霉MTCC 557生产CyA的影响。发酵在25±2℃下进行9天。与优化前的792±33mg/kg底物相比，水解麦麸粉和椰子油饼（1:1）的组合在初始水分含量为70%时，支持最大产量为3872±156mg CyA/kg底物。此外，添加盐、甘油（1%w/w）和硫酸铵（1%w/w）可使CyA产量提高到5454±75mg/kg底物。用6ml 72小时龄的种子培养物接种5g固体底物，可使CyA的最大产量达到6480±95mg/kg底物。
