Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Linz, Vienna, Austria.
J Tissue Eng Regen Med. 2010 Mar;4(3):242-5. doi: 10.1002/term.236.
The umbilical cord matrix as well as liposuction material have been demonstrated to contain cells capable of differentiating towards the mesodermal lineage. High availability and low donor site morbidity appear promising for the use of human umbilical cord matrix cells (HUCMs) and adipose-derived stem cells (ASCs) in cell-based therapies. In the present study we focused on cartilage regeneration and compared HUCMs and ASCs regarding their potential to differentiate towards the chondrogenic lineage. Cells were isolated by explantation culture or enzymatic digestion, phenotypically characterized by flow cytometry and differentiated as 3D micromass pellets for up to 35 days. Under tested conditions, ASCs demonstrated significantly higher glycosaminoglycan synthesis compared to HUCMs. qRT-PCR data gave evidence that chondrogenic genes are expressed by both ASCs and HUCMs. However, higher expression levels of ASCs suggest that this cell type has higher potential for differentiation towards a cartilage-like phenotype than HUCMs. In conclusion, both cell types, HUCMs and ASCs, are easily available, possess typical properties of mesenchymal stem cells and are thus promising for cell-based therapies. However, in terms of cartilage regeneration, ASCs might be more suitable than HUCMs.
脐带基质和脂肪抽吸物已被证明含有能够向中胚层谱系分化的细胞。人脐带基质细胞（HUCMs）和脂肪来源干细胞（ASCs）在基于细胞的治疗中具有高可用性和低供体部位发病率的优势。在本研究中，我们专注于软骨再生，并比较了 HUCMs 和 ASCs 向软骨谱系分化的潜力。细胞通过组织块培养或酶消化分离，通过流式细胞术进行表型鉴定，并在 3D 微团中分化长达 35 天。在测试条件下，ASCs 的糖胺聚糖合成明显高于 HUCMs。qRT-PCR 数据表明，两种细胞类型（ASCs 和 HUCMs）都表达软骨形成基因。然而，ASCs 的表达水平更高，表明该细胞类型向软骨样表型分化的潜力高于 HUCMs。总之，两种细胞类型，HUCMs 和 ASCs，都很容易获得，具有间充质干细胞的典型特性，因此是基于细胞的治疗的有前途的选择。然而，就软骨再生而言，ASCs 可能比 HUCMs 更合适。
