鉴定人肠道平滑肌间质细胞中的 TRPM7 通道。
Identification of TRPM7 channels in human intestinal interstitial cells of Cajal.
机构信息
School of Korean Medicine, Pusan National University, Yangsan 626-870, South Korea.
出版信息
World J Gastroenterol. 2009 Dec 14;15(46):5799-804. doi: 10.3748/wjg.15.5799.
Abstract
AIM
To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract.
METHODS
Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs).
RESULTS
The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La(3+), TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue.
CONCLUSION
These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves.
摘要
目的
研究人类胃肠道（GI）中慢电波的特征和瞬时受体电位 melastatin 型 7（TRPM7）的存在。
方法
使用常规微电极技术记录来自人类 GI 平滑肌组织的细胞内电反应。免疫组织化学用于鉴定 Cajal 间质细胞（ICCs）中的 TRPM7 通道。
结果
人类胃肠道产生慢电波，并且 ICCs 作为起搏细胞发挥作用。非选择性阳离子通道阻滞剂氟芬那酸（Flufenamic acid）以及 2-APB（2-氨基乙氧基二苯硼酸盐）和 La（3+），TRPM7 通道阻滞剂，抑制慢电波。此外，TRPM7 通道在人类组织中的 ICCs 中表达。
结论
这些结果表明，人类胃肠道产生慢电波，并且 ICCs 中表达的 TRPM7 通道可能参与慢电波的产生。
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本文引用的文献
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3
Suppression of transient receptor potential melastatin 7 channel induces cell death in gastric cancer.
Cancer Sci. 2008 Dec;99(12):2502-9. doi: 10.1111/j.1349-7006.2008.00982.x. Epub 2008 Nov 20.
4
TRPM7 ion channels are required for sustained phosphoinositide 3-kinase signaling in lymphocytes.
Cell Metab. 2008 Jul;8(1):84-93. doi: 10.1016/j.cmet.2008.06.002.
5
TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane.
Proc Natl Acad Sci U S A. 2008 Jun 17;105(24):8304-8. doi: 10.1073/pnas.0800881105. Epub 2008 Jun 6.
6
The mechanism and spread of pacemaker activity through myenteric interstitial cells of Cajal in human small intestine.
Gastroenterology. 2007 May;132(5):1852-65. doi: 10.1053/j.gastro.2007.02.049. Epub 2007 Feb 23.
7
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9
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