Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauer Str. 108, 01307 Dresden, Germany.
Biomacromolecules. 2010 Jan 11;11(1):233-7. doi: 10.1021/bm901082y.
We report on the protein-resistant properties of glass substrates coated with novel copolymers of 2-aminoethyl methacrylate hydrochloride and poly(ethylene glycol) methyl ether methacrylate (AEM-PEG). In comparison to currently available protein-blocking polymer systems, such as poly-l-lysine-poly(ethylene glycol), silane-based poly(ethylene glycol), and poly(ethylene glycol) brushes prepared by surface-initiated polymerization, the proposed AEM-PEG offers the combined advantages of low cost, simplicity of use, and applicability in aqueous solutions. We demonstrate the capability of AEM-PEG to block the surface binding of globular proteins (tubulin), their assemblies (microtubules), and functional motor proteins (kinesin-1). Moreover, we demonstrate the applicability of AEM-PEG for surface patterning of proteins in microfluidic devices.
我们报告了经新型 2-氨基乙基甲基丙烯酸盐酸盐和聚乙二醇甲基醚甲基丙烯酸酯（AEM-PEG）共聚物修饰的玻璃基底的抗蛋白特性。与目前可用的蛋白阻断聚合物系统（如聚赖氨酸-聚乙二醇、硅烷基聚乙二醇和通过表面引发聚合制备的聚乙二醇刷）相比，所提出的 AEM-PEG 具有成本低、使用简单以及适用于水溶液的综合优势。我们证明了 AEM-PEG 能够阻止球状蛋白（微管蛋白）、它们的组装体（微管）和功能性马达蛋白（驱动蛋白-1）在表面的结合。此外，我们还证明了 AEM-PEG 可用于微流控装置中蛋白质的表面图案化。
