Ghogare Ashwini A, Miller Joann M, Mondal Bikash, Lyons Alan M, Cengel Keith A, Busch Theresa M, Greer Alexander
Department of Chemistry and Graduate Center, Brooklyn College, City University of New York, Brooklyn, NY.
Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA.
Photochem Photobiol. 2016 Jan-Feb;92(1):166-72. doi: 10.1111/php.12538. Epub 2015 Nov 4.
We describe progress on a one-step photodynamic therapy (PDT) technique that is simple: device tip delivery of sensitizer, oxygen and light simultaneously. Control is essential for their delivery to target sites to generate singlet oxygen. One potential problem is the silica device tip may suffer from biomaterial fouling and the pace of sensitizer photorelease is slowed. Here, we have used biomaterial (e.g. proteins, cells, etc.) from SQ20B head and neck tumors and whole blood for an assessment of fouling of the silica tips by adsorption. It was shown that by exchanging the native silica tip for a fluorinated tip, a better nonstick property led to an increased sensitizer output by ~10%. The fluorinated tip gave a sigmoidal photorelease where singlet oxygen is stabilized to physical quenching, whereas the native silica tip with unprotected SiO-H groups gave a slower (pseudolinear) photorelease. A further benefit from fluorinated silica is that 15% less biomaterial adheres to its surface compared to native silica based on a bicinchoninic acid assay (BCA) and X-ray photoelectron spectroscopy (XPS) measurements. We discuss how the fluorination of the device tip increases biofouling resistance and can contribute to a new pointsource PDT tool.
我们描述了一种一步式光动力疗法（PDT）技术的进展，该技术很简单：在设备尖端同时输送敏化剂、氧气和光。对它们输送到靶位点以产生单线态氧来说，控制至关重要。一个潜在问题是二氧化硅设备尖端可能会受到生物材料污染，敏化剂光释放的速度会减慢。在这里，我们使用了来自SQ20B头颈肿瘤的生物材料（如蛋白质、细胞等）和全血，通过吸附来评估二氧化硅尖端的污染情况。结果表明，用氟化尖端替换天然二氧化硅尖端，更好的不粘性能使敏化剂输出增加了约10%。氟化尖端呈现出S形光释放，其中单线态氧通过物理猝灭得以稳定，而带有未受保护的Si - O - H基团的天然二氧化硅尖端则呈现出较慢的（准线性）光释放。基于二辛可宁酸测定（BCA）和X射线光电子能谱（XPS）测量，氟化二氧化硅的另一个优点是与天然二氧化硅相比，其表面附着的生物材料减少了15%。我们讨论了设备尖端的氟化如何增加抗生物污染能力，并有助于形成一种新的点源PDT工具。
