Division of Biomaterials Engineering, Kangwon National University, Medical and Bio-Material Research Center, Chuncheon, Gangwon 200-701, Republic of Korea.
J Agric Food Chem. 2010 Feb 10;58(3):1719-25. doi: 10.1021/jf903493b.
This study was designed to evaluate the combined effects of probiotic fermentation and high-pressure extraction (HPE) on the functional properties of Codonopsis lanceolata. The ground C. lanceolata samples were anaerobically fermented with Lactobacillus acidophilus ADH, Bifidobacterium longum B6, Lactobacillus rhamnosus GG, or Lactobacillus paracasei at 37 degrees C for 10 days and subjected to 500 MPa at 50 degrees C for 30 min. The extraction yields of C. lanceolata samples were noticeably increased to 29-32% by HPE. The B. longum-fermented C. lanceolata samples extracted by high pressure (BLF-HPE) exhibited the highest antimicrobial activity (MIC < 14 mg/mL) against Listeria monocytogenes, Staphylococcus aureus, Shigella boydii, and Salmonella typhimurium. The nonfermented C. lanceolata samples extracted with high pressure (NF-HPE) had the highest total phenolic content (13.3 mg of GAE/g). The lowest effective concentrations (EC(50) and EC(0.5)) were 4.55 and 1.76 mg/mL, respectively, for NF-HPE extracts, indicating its highest antioxidant activity. The BLF-HPE and L. rhamnosus-fermented C. lanceolata samples extracted by high pressure (LRF-HPE) exhibited the highest antimutagenic activities in S. typhimurium TA 100, which were 82 and 83% inhibition, respectively. The use of probiotic fermentation and HPE can produce more biologically active compounds in C. lanceolata than the conventional solvent extraction method. The results provide pharmaceutically useful information for improving biological properties and an approach to drug discovery.
这项研究旨在评估益生菌发酵和高压提取（HPE）对党参功能特性的综合影响。将党参粉末样品在 37°C 下用嗜酸乳杆菌 ADH、长双歧杆菌 B6、鼠李糖乳杆菌 GG 或副干酪乳杆菌厌氧发酵 10 天，然后在 50°C 下用 500 MPa 处理 30 分钟。HPE 可使党参样品的提取率显著提高到 29-32%。高压提取的长双歧杆菌发酵党参样品（BLF-HPE）对单核细胞增生李斯特菌、金黄色葡萄球菌、福氏志贺菌和鼠伤寒沙门氏菌表现出最高的抗菌活性（MIC<14mg/mL）。高压提取的未发酵党参样品（NF-HPE）具有最高的总酚含量（13.3mg GAE/g）。NF-HPE 提取物的最低有效浓度（EC(50)和 EC(0.5)）分别为 4.55 和 1.76mg/mL，表明其具有最高的抗氧化活性。BLF-HPE 和高压提取的鼠李糖乳杆菌发酵党参样品（LRF-HPE）在鼠伤寒沙门氏菌 TA100 中表现出最高的抗突变活性，分别为 82%和 83%的抑制率。与传统溶剂提取方法相比，益生菌发酵和 HPE 可在党参中产生更多具有生物活性的化合物。研究结果为提高党参的生物特性提供了有价值的药用信息，并为药物发现提供了一种方法。
