Enteric Virus Laboratory, Department of Microbiology, School of Biology, University of Barcelona, 08028, Barcelona, Spain.
Environ Sci Technol. 2010 Jan 15;44(2):808-12. doi: 10.1021/es9018964.
The infectivity evaluation of noncultivatable viruses, such as human norovirus, is crucial to address needs for ensuring the safety in usage of water and marine products. In this work, we tested a new approach to evaluate viral particle integrity, in which oxidatively produced carbonyl groups on viral capsid protein were quantitatively detected. As a result, the decrease in the infectivity of human astrovirus, a representative enteric virus, positively correlated with the amount of oxidative damage on viral particles. Furthermore, when human norovirus was treated by 1 ppm free chlorine for 15 min, 49.93% of virions were recovered as oxidatively damaged particles, which represents a 5-fold increase over those treated by 0.5 ppm free chlorine for 15 min. The detection of the carbonylated viral particles could be a powerful tool for the evaluation of the decrease in the infectivity of noncultivatable viruses.
不可培养病毒（如人类诺如病毒）的感染力评估对于确保水和海产品使用安全至关重要。在这项工作中，我们测试了一种评估病毒粒子完整性的新方法，其中定量检测了病毒衣壳蛋白上氧化产生的羰基。结果表明，代表性肠道病毒人类星状病毒的感染力下降与病毒粒子上的氧化损伤量呈正相关。此外，当人类诺如病毒用 1 ppm 的游离氯处理 15 分钟时，49.93%的病毒粒子被回收为氧化损伤颗粒，比用 0.5 ppm 的游离氯处理 15 分钟增加了 5 倍。检测羰基化的病毒颗粒可能是评估不可培养病毒感染力下降的有力工具。
