Luehrsen K R, Walbot V
Department of Biological Sciences, Stanford University, CA 94305.
Mol Gen Genet. 1991 Jan;225(1):81-93. doi: 10.1007/BF00282645.
The inclusion of the alcohol dehydrogenase 1-S(Adh 1-S) intron 1 in the transcription unit of maize gene constructs has been shown to increase gene expression in cultured maize cells. We have extended these studies with Adh1-S intron 1 using the firefly luciferase, Escherichia coli beta-glucuronidase and chloramphenicol acetyltransferase reporter genes adjoined to different plant promoters and find enhancement of transient gene expression in all cases but one. We also show that the enhancement phenomenon can be mediated by the third intron of the maize actin gene. In all cases tested, the inclusion of an intron results in increased levels of steady-state RNA. The degree of enhancement depends on the exon sequences flanking the intron; flanking exons also influence the efficiency of intron splicing. Unexpectedly, unspliced RNAs accumulate during the transient assay.
已证明在玉米基因构建体的转录单元中包含乙醇脱氢酶1-S（Adh 1-S）内含子1可提高培养的玉米细胞中的基因表达。我们使用萤火虫荧光素酶、大肠杆菌β-葡萄糖醛酸酶和氯霉素乙酰转移酶报告基因，将其与不同的植物启动子相连，对Adh1-S内含子1进行了这些研究的扩展，发现除了一种情况外，在所有情况下瞬时基因表达均得到增强。我们还表明，增强现象可由玉米肌动蛋白基因的第三个内含子介导。在所有测试的情况下，内含子的包含导致稳态RNA水平增加。增强程度取决于内含子两侧的外显子序列；侧翼外显子也影响内含子剪接的效率。出乎意料的是，在瞬时测定过程中未剪接的RNA会积累。
