Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Foodborne Pathog Dis. 2010 Apr;7(4):383-90. doi: 10.1089/fpd.2009.0358.
The occurrence of Salmonella in 750 birds was assessed. The samples included the internal organs (caecal pouches, yolk sac, liver, and lung) of imported 1-day-old chicks (n = 150), grandparent chicks (n = 150), breeder chicks (n = 150), ducklings (n = 150), and turkey poults (n = 150), and paper-lined boxes (n = 250). Salmonellae isolated from the internal organs and paper-lined box of 1-day-old chicks, ducklings, and poults were mostly evident from the paper-lined box followed by caecal samples. Imported 1-day-old grandparent flocks were Salmonella free. Although 23.3% of the imported breeder flocks were positive for Salmonella, the imported duckling flocks and day-old turkey poults exhibited 19.3% and 12.6%, respectively. The widest diversity in isolated salmonellae was from the 1-day-old chicks where Salmonella Newport, Salmonella Kentucky, Salmonella Enteritidis, Salmonella Shubra, Salmonella Saintpaul, and Salmonella Agona were isolated. On the other hand, two Salmonella serovars were isolated from the imported breeders, Salmonella Shubra and Salmonella Shipley, and from the imported ducklings, Salmonella Shubra and Salmonella Saintpaul. The three Salmonella serovars isolated from the imported day-old turkey poults were Salmonella Shubra, Salmonella Newport, and Salmonella Saintpaul. The high percentage and diversity of Salmonella isolation from the imported birds cause concern because of the zoonotic potential of this agent and its economical importance to the local commercial poultry breeding industry. From 80 samples investigated for Salmonella, the positivity of the standard microbiological technique method was 17.5% and of the polymerase chain reaction method (Salmonella-specific invA gene) was 22.5%. The concordance between the two methods was 90% (k = 0.850). Our results indicated that the polymerase chain reaction approach is better than culturing for detecting Salmonella in poultry samples when using the preenriched medium combinations used in this study.
本研究评估了 750 只禽类中沙门氏菌的发生情况。样本包括进口 1 日龄雏鸡（n=150）、祖代鸡（n=150）、种鸡（n=150）、鸭（n=150）和火鸡雏鸡（n=150）的内脏器官（盲肠囊、蛋黄囊、肝脏和肺），以及纸质衬盒（n=250）。从 1 日龄雏鸡、鸭和火鸡雏鸡的内脏器官和纸质衬盒中分离出的沙门氏菌，主要来源于纸质衬盒，其次是盲肠样本。进口的 1 日龄祖代鸡群未检出沙门氏菌。尽管 23.3%的进口种鸡群呈沙门氏菌阳性，但进口鸭群和 1 日龄火鸡雏鸡的阳性率分别为 19.3%和 12.6%。从 1 日龄雏鸡中分离出的沙门氏菌多样性最广，包括沙门氏菌纽波特、沙门氏菌肯塔基、沙门氏菌肠炎、沙门氏菌舒布拉、沙门氏菌圣保罗和沙门氏菌阿贡纳。另一方面，从进口种鸡中分离到两种沙门氏菌血清型，即沙门氏菌舒布拉和沙门氏菌希普利，从进口鸭中分离到沙门氏菌舒布拉和沙门氏菌圣保罗。从进口 1 日龄火鸡雏鸡中分离到的三种沙门氏菌血清型为沙门氏菌舒布拉、沙门氏菌纽波特和沙门氏菌圣保罗。从进口禽类中分离出的沙门氏菌数量和多样性较高，令人担忧，因为这种病原体具有人畜共患的潜力，对当地商业家禽养殖行业具有重要的经济意义。在对 80 份沙门氏菌样本进行检测时，标准微生物学技术方法的阳性率为 17.5%，聚合酶链反应方法（沙门氏菌特异性 invA 基因）的阳性率为 22.5%。两种方法的一致性为 90%（k=0.850）。我们的结果表明，当使用本研究中使用的预富集培养基组合时，聚合酶链反应方法比培养法更适合检测家禽样本中的沙门氏菌。
