分析蛋白激酶 Ceta 在人肝癌中的表达。
Analysing the expression of protein kinase C eta in human hepatocellular carcinoma.
机构信息
Departments of Laboratory Medicine and Clinical Research, Kaohsiung Medical University Hospital, Institute of Clinical Research, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
Pathology. 2009;41(7):626-9. doi: 10.3109/00313020903273076.
AIMS
Protein kinase Cs (PKCs) play important roles in cell proliferation, differentiation, apoptosis, migration and tumorigenesis. In this report, we investigated the expression of PKCeta in human hepatocellular carcinoma (HCC) tissues and explored its role in the development of HCC.
METHODS
We used real-time quantitative RT-PCR, mutation analysis, and immunohistochemical staining to analyse the expression of PKCeta in 50 pairs of human hepatocellular carcinoma (HCC) tissues.
RESULTS
Expression of PKCeta was down-regulated in 82% of HCC tissues and the reduction of PKCeta was associated with poorer long-term survival of HCC patients.
CONCLUSION
Reduced expression of PKCeta may represent a molecular lesion in the development of more aggressive disease of HCC.
目的
蛋白激酶 C（PKC）在细胞增殖、分化、凋亡、迁移和肿瘤发生中发挥重要作用。在本报告中，我们研究了 PKCeta 在人肝细胞癌（HCC）组织中的表达，并探讨了其在 HCC 发展中的作用。
方法
我们使用实时定量 RT-PCR、突变分析和免疫组织化学染色来分析 50 对人肝细胞癌（HCC）组织中 PKCeta 的表达。
结果
PKCeta 的表达在 82%的 HCC 组织中下调，PKCeta 的减少与 HCC 患者的长期生存不良相关。
结论
PKCeta 的表达降低可能代表 HCC 更具侵袭性疾病发展中的分子病变。
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