用于鉴别派杰细胞与托克尔细胞的有用免疫组织化学标志物。

Useful immunohistochemical markers for distinguishing Paget cells from Toker cells.

机构信息

Department of Pathology, Gil Medical Center, Gachon University, School of Medicine and Science, Incheon, Korea.

出版信息

Pathology. 2009;41(7):640-4. doi: 10.3109/00313020903273092.

DOI:10.3109/00313020903273092

PMID:20001343

Abstract

AIMS

Toker cells (TCs) are normal components of the nipple epidermis. Because of their morphological and immunohistochemical similarities to mammary Paget cells (PCs), their presence may lead to a mistaken diagnosis of Paget's disease. The objective of this study was to identify immunohistochemical markers that are able to distinguish TCs from mammary PCs.

METHODS

Nipple tissue, derived from surgical specimens following radical mastectomy for breast cancer, was obtained from 30 cases diagnosed with TCs and 30 cases of mammary Paget's disease (MPD). The tissue was examined by immunohistochemistry using antibodies to c-erbB-2, cytokeratin (CK)7, Ki-67 and oestrogen receptor (ER).

RESULTS

TCs were consistently positive for CK7 and ER but negative for c-erbB-2. TCs either did not express Ki-67 or expressed very low levels with only 1-3 positive cells per high-power field. In contrast, MPD tended strongly to express both Ki-67 and c-erbB-2.

CONCLUSIONS

Results suggested that TCs and mammary PCs differ substantially in their expression of ER, c-erbB-2 and Ki-67, although they share immunoreactivity to CK7. Both Ki-67 and c-erbB-2 were consistently positive in mammary PCs but almost always negative in TCs, while ER was consistently positive in TCs. Therefore, a combination of these markers may allow TCs to be distinguished from mammary PCs.

摘要

目的

Toker 细胞（TCs）是乳头表皮的正常组成部分。由于其形态学和免疫组织化学特征与乳腺 Paget 细胞（PCs）相似，因此它们的存在可能导致对 Paget 病的误诊。本研究的目的是确定能够区分 TCs 和乳腺 PCs 的免疫组织化学标志物。

方法

从因乳腺癌根治性乳房切除术而获得的 30 例 TCs 和 30 例乳腺 Paget 病（MPD）的手术标本中获得乳头组织。使用针对 c-erbB-2、细胞角蛋白（CK）7、Ki-67 和雌激素受体（ER）的抗体对组织进行免疫组织化学检查。

结果

TCs 始终对 CK7 和 ER 呈阳性，但对 c-erbB-2 呈阴性。TCs 要么不表达 Ki-67，要么仅表达 1-3 个阳性细胞/高倍视野，表达水平非常低。相比之下，MPD 强烈倾向于表达 Ki-67 和 c-erbB-2。

结论

结果表明，TCs 和乳腺 PCs 在 ER、c-erbB-2 和 Ki-67 的表达上存在显著差异，尽管它们对 CK7 的免疫反应性相似。Ki-67 和 c-erbB-2 在乳腺 PCs 中始终呈阳性，但在 TCs 中几乎总是阴性，而 ER 在 TCs 中始终呈阳性。因此，这些标志物的组合可能允许将 TCs 与乳腺 PCs 区分开来。