National Nanotechnology Center, National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand.
J Microencapsul. 2010;27(5):436-46. doi: 10.3109/02652040903447686.
The objective of this work has been the microencapsulation of Asiatic Pennywort (AP) extract with lecithin from soybean. The effect of various quantities of non-ionic surfactant (Montanov82) on liposomes upon physicochemical characteristics as well as their in vitro bio-activities was investigated. An addition of surfactant resulted in a decrease in particle size and an increase in percentage AP entrapment efficiency of liposomes. The surfactant-loaded liposomes demonstrated higher stability than surfactant-free liposomes where higher percentage AP remaining of liposomes can be achieved depending on surfactant concentration. No significant difference was found on AP release profiles among varied surfactant concentrations, although a presence of surfactant resulted in prolonged AP release rate. Liposomal AP with 20% w/w surfactant or higher demonstrated low cytotoxicity, a stronger anti-oxidation effect and collagen production on dermal fibroblast cells when compared with free AP and surfactant-free liposomes, possibly due to better cell internalization and less AP degradation in cells.
本工作的目的是用大豆卵磷脂对贯叶金丝桃（AP）提取物进行微胶囊化。研究了不同量的非离子表面活性剂（Montanov82）对脂质体理化特性及其体外生物活性的影响。添加表面活性剂会导致脂质体的粒径减小，AP 的包封效率增加。与无表面活性剂的脂质体相比，负载表面活性剂的脂质体具有更高的稳定性，根据表面活性剂的浓度，可以实现更高比例的 AP 保留在脂质体中。尽管表面活性剂的存在会延长 AP 的释放速率，但在不同表面活性剂浓度下，AP 的释放曲线没有明显差异。与游离 AP 和无表面活性剂的脂质体相比，含有 20%w/w 表面活性剂或更高浓度表面活性剂的 AP 脂质体对真皮成纤维细胞表现出较低的细胞毒性、更强的抗氧化作用和胶原蛋白产生，这可能是由于更好的细胞内化和细胞内 AP 降解减少。
