红细胞中的 O-β-N-乙酰-D-氨基葡萄糖苷酶与意大利空军特技飞行员。
O-beta-N-acetyl-D-glucosaminidase in erythrocytes of Italian air force acrobatic pilots.
机构信息
Department of Human Morphology and Biomedical Sciences Città Studi, Laboratory of Clinical Pathology, University of Milan, Milan, Italy.
出版信息
Clin Chem Lab Med. 2010 Feb;48(2):213-6. doi: 10.1515/CCLM.2010.051.
BACKGROUND
Italian air force acrobatic pilots are occupationally susceptible to oxidative stress damage that can lead to overt signs and symptoms of hypoxia. We propose erythrocyte glycohydrolases as new, sensitive markers to assess oxidative stress.
METHODS
We measured erythrocyte concentrations of beta-D-glucuronidase (GCR), hexosaminidase, O-beta-N-acetyl-D-glucosaminidase (O-GlcNAcase), plasma membrane fluidity and plasma hydroperoxides from 19 pilots and compared these to 40 matched healthy subjects.
RESULTS
Plasma hydroperoxide concentrations and the erythrocyte ghosts' fluorescence anisotropy were significantly lower in the pilots. Concentrations of GCR, O-GlcNAcase and hexosaminidase in pilots were significantly different from controls, being lower, higher and higher, respectively.
CONCLUSIONS
Pilots, in spite of their oxidative stress, are better protected than controls, probably as a result of their physical training and proper diet. Our results confirm that erythrocytes, with their 120-day life span, are a useful model for investigating physiopathological conditions, and glycohydrolases are good markers for monitoring oxidative stress, even in healthy people.
背景
意大利空军特技飞行员容易受到氧化应激损伤，这可能导致明显的缺氧迹象和症状。我们提出红细胞糖基水解酶作为评估氧化应激的新的敏感标志物。
方法
我们测量了 19 名飞行员的红细胞β-D-葡糖醛酸酶（GCR）、己糖胺酶、O-β-N-乙酰-D-葡糖胺酶（O-GlcNAcase）浓度、血浆膜流动性和血浆过氧化物，并将这些与 40 名匹配的健康受试者进行了比较。
结果
飞行员的血浆过氧化物浓度和红细胞胞浆荧光各向异性明显较低。飞行员的 GCR、O-GlcNAcase 和己糖胺酶浓度与对照组有显著差异，分别较低、较高和更高。
结论
尽管飞行员面临氧化应激，但他们受到的保护比对照组更好，这可能是由于他们的体能训练和适当的饮食。我们的结果证实，红细胞具有 120 天的寿命，是研究生理病理状况的有用模型，糖基水解酶是监测氧化应激的良好标志物，即使在健康人群中也是如此。
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