Department of Colorectal Surgery, Cancer Hospital, Fudan University, Shanghai 200032, People's Republic of China.
Biosci Rep. 2010 Dec;30(6):383-90. doi: 10.1042/BSR20090129.
The role of RGS2 (regulator of G-protein signalling 2) has been studied in several tumours. The purpose of the present study is to investigate the correlations between clinicopathological factors and patients' survival time and RGS2 expression in stage II and III CRC (colorectal cancer) patients. Real-time quantitative PCR was performed in 36 CRC tissues with recurrence and 28 without recurrence, and in three CRC-metastasis-derived cell lines (SW620, LoVo and Colo205) and 3 primary-CRC-derived ones (SW480, Caco-2 and HCT116) to examine RGS2 mRNA expression. In addition, to provide visualized evidence for RGS2 mRNA expression, random CRC samples were also performed with RT-PCR (reverse transcription-PCR). RGS2 protein was detected by immunostaining in 118 paraffin-embedded specimens, and the correlations between clinicopathological factors and survival time and RGS2 expression were analysed. We found that RGS2 mRNA was down-regulated both in CRC tissues with recurrence and metastasis-derived cell lines, and the expression level of RGS2 was unrelated to gender, age, tumour grade, or lymphovascular or perineural invasion. However, it was positively related to disease-free survival time (P<0.05). Furthermore, low RGS2 expression indicated a poorer survival rate (P<0.05, log-rank test). Multivariate analysis also showed that weak RGS2 protein expression was an independent adverse prognosticator in CRC (P<0.05). Taken together, we suggested that down-regulation of RGS2 might play an important role in CRC metastasis and predict poor prognosis in stage II and III CRC patients.
RGS2（G 蛋白信号调节因子 2）的作用已在多种肿瘤中进行了研究。本研究旨在探讨 RGS2 在 II 期和 III 期 CRC（结直肠癌）患者的临床病理因素与患者生存时间之间的相关性。在 36 例有复发和 28 例无复发的 CRC 组织中进行了实时定量 PCR，并在 3 个 CRC 转移衍生细胞系（SW620、LoVo 和 Colo205）和 3 个原发性 CRC 衍生细胞系（SW480、Caco-2 和 HCT116）中检测 RGS2 mRNA 表达。此外，为了提供 RGS2 mRNA 表达的可视化证据，还对随机 CRC 样本进行了 RT-PCR（逆转录-PCR）。用免疫染色法检测了 118 例石蜡包埋标本中的 RGS2 蛋白，并分析了临床病理因素与生存时间和 RGS2 表达之间的相关性。我们发现，RGS2 mRNA 在有复发和转移的 CRC 组织和细胞系中均下调，RGS2 的表达水平与性别、年龄、肿瘤分级或血管淋巴管或神经周围浸润无关。然而，它与无病生存时间呈正相关（P<0.05）。此外，低 RGS2 表达预示着生存率较差（P<0.05，对数秩检验）。多变量分析还表明，RGS2 蛋白表达弱是 CRC 的独立不良预后因素（P<0.05）。综上所述，我们认为 RGS2 的下调可能在 CRC 转移中起重要作用，并预测 II 期和 III 期 CRC 患者的预后不良。
