School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
Environ Microbiol. 2010 Mar;12(3):689-703. doi: 10.1111/j.1462-2920.2009.02111.x. Epub 2009 Dec 4.
The objective of this study was to examine the presence and diversity of Archaea within mineral and ornithogenic soils from 12 locations across the Ross Sea region. Archaea were not abundant but DNA sufficient for producing 16S rRNA gene clone libraries was extracted from 18 of 51 soil samples, from four locations. A total of 1452 clones were analysed by restriction fragment length polymorphism and assigned to 43 operational taxonomic units from which representatives were sequenced. Archaea were primarily restricted to coastal mineral soils which showed a predominance of Crenarchaeota belonging to group 1.1b (> 99% of clones). These clones were assigned to six clusters (A through F), based on shared identity to sequences in the GenBank database. Ordination indicated that soil chemistry and water content determined archaeal community structure. This is the first comprehensive study of the archaeal community in Antarctic soils and as such provides a reference point for further investigation of microbial function in this environment.
本研究旨在检测罗斯海地区 12 个地点的矿物和鸟粪土壤中古菌的存在和多样性。古菌并不丰富，但从四个地点的 51 个土壤样本中提取了足够用于产生 16S rRNA 基因克隆文库的 DNA。通过限制性片段长度多态性分析了总共 1452 个克隆，并将其分配到 43 个操作分类单元，其中代表序列进行了测序。古菌主要局限于沿海矿物土壤，其中以属于第 1.1b 组（> 99%的克隆）的 Crenarchaeota 为主。这些克隆基于与 GenBank 数据库中的序列的共享同一性，被分配到六个聚类（A 到 F）中。排序表明土壤化学和含水量决定了古菌群落结构。这是对南极土壤中古菌群落的首次全面研究，因此为进一步研究该环境中的微生物功能提供了参考点。
