Li Qinxin, Wang Nengfei, Han Wenbing, Zhang Botao, Zang Jiaye, Qin Yiling, Wang Long, Liu Jie, Zhang Tao
College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.
Biology (Basel). 2022 Dec 19;11(12):1855. doi: 10.3390/biology11121855.
It is believed that polar regions are influenced by global warming more significantly, and because polar regions are less affected by human activities, they have certain reference values for future predictions. This study aimed to investigate the effects of climate warming on soil microbial communities in lake areas, taking Kitezh Lake, Antarctica as the research area. Below-peak soil, intertidal soil, and sediment were taken at the sampling sites, and we hypothesized that the diversity and composition of the bacterial and archaeal communities were different among the three sampling sites. Through 16S rDNA sequencing and analysis, bacteria and archaea with high abundance were obtained. Based on canonical correspondence analysis and redundancy analysis, pH and phosphate had a great influence on the bacterial community whereas pH and nitrite had a great influence on the archaeal community. Weighted gene coexpression network analysis was used to find the hub bacteria and archaea related to geochemical factors. The results showed that in addition to pH, phosphate, and nitrite, moisture content, ammonium, nitrate, and total carbon content also play important roles in microbial diversity and structure at different sites by changing the abundance of some key microbiota.
人们认为，极地地区受全球变暖的影响更为显著，而且由于极地地区受人类活动的影响较小，它们对未来预测具有一定的参考价值。本研究旨在以南极洲的基捷日湖为研究区域，调查气候变暖对湖区土壤微生物群落的影响。在采样点采集了湖底土壤、潮间带土壤和沉积物，我们假设三个采样点的细菌和古菌群落的多样性和组成存在差异。通过16S rDNA测序和分析，获得了高丰度的细菌和古菌。基于典范对应分析和冗余分析，pH值和磷酸盐对细菌群落有很大影响，而pH值和亚硝酸盐对古菌群落有很大影响。采用加权基因共表达网络分析来寻找与地球化学因素相关的关键细菌和古菌。结果表明，除了pH值、磷酸盐和亚硝酸盐外，水分含量、铵、硝酸盐和总碳含量也通过改变一些关键微生物群的丰度，在不同位点的微生物多样性和结构中发挥重要作用。
