Exp Dermatol. 2010 Aug;19(8):e310-3. doi: 10.1111/j.1600-0625.2009.01008.x.
Activating transcription factor 3 (ATF3) expression is increased in erythema multiforme and is regulated by IFN-gamma in human keratinocytes. Experimental Dermatology 2010; 19: e310-e313. Abstract: Activating transcription factor 3 (ATF3) is a member of the ATF/cyclic AMP responsive element-binding protein (CREB) family of transcription factors and is involved in the regulation of immune responses, apoptosis, DNA repair and oncogenesis. The epidermal expression of ATF3 in the setting of cutaneous inflammation has not been well characterized. To examine the expression of ATF3 in the setting of inflammatory skin disease, ATF3 protein expression was analysed by immunohistochemistry (IHC). We found diffuse epidermal ATF3 protein expression in skin biopsies of erythema multiforme (EM). Given the role of interferon (IFN)-gamma in erythema multiforme, we sought to examine the impact of IFN-gamma on ATF3 expression in human keratinocytes. IFN-gamma induced ATF3 mRNA and protein in primary human keratinocytes and HaCaT cells. Thus, epidermal ATF3 expression can be increased in the setting of inflammatory skin diseases and is regulated by IFN-gamma in human keratinocytes.
激活转录因子 3（ATF3）在多形性红斑中的表达增加，并在人类角质形成细胞中受 IFN-γ调节。实验皮肤病学 2010；19: e310-e313. 摘要：激活转录因子 3（ATF3）是 ATF/cAMP 反应元件结合蛋白（CREB）家族转录因子的成员，参与免疫反应、细胞凋亡、DNA 修复和肿瘤发生的调节。表皮中 ATF3 的表达在皮肤炎症环境中尚未得到很好的描述。为了研究 ATF3 在炎症性皮肤病中的表达，我们通过免疫组织化学（IHC）分析了 ATF3 蛋白的表达。我们发现多形性红斑（EM）皮肤活检中存在弥漫性表皮 ATF3 蛋白表达。鉴于干扰素（IFN）-γ在多形性红斑中的作用，我们试图研究 IFN-γ对人角质形成细胞中 ATF3 表达的影响。IFN-γ 在原代人角质形成细胞和 HaCaT 细胞中诱导 ATF3 mRNA 和蛋白的表达。因此，表皮 ATF3 的表达可以在炎症性皮肤病的情况下增加，并在人类角质形成细胞中受 IFN-γ调节。
