Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Virginia Beach, VA 23455-3363, USA.
FEMS Microbiol Lett. 2010 Feb;303(1):55-60. doi: 10.1111/j.1574-6968.2009.01861.x. Epub 2009 Nov 23.
The frequent coisolation of bacteria with Phytophthora and Pythium species suggests possible interspecies communication. Zoospore-free fluids (ZFF) from bacteria-free and nutrient-depleted zoospore suspensions were examined to investigate the production of autoinducer-2 (AI-2), a bacterial interspecies signal molecule, by zoosporic oomycetes. ZFF from Phytophthora nicotianae, Phytophthora sojae, and Pythium aphanidermatum triggered luminescence of the Vibrio harve7yi AI-2 reporter, indicating the presence of AI-2 in zoospore extracellular products and the potential of cross-kingdom communication between oomycetes and bacteria. The production of AI-2 by zoospores was confirmed by chemical assays. These results provide a new insight into the physiology and ecology of oomycetes.
细菌与卵菌和绵霉菌频繁共分离提示可能存在种间交流。从无细菌和营养耗尽的游动孢子悬浮液中分离出的游动孢子无游离液(ZFF),以研究种间信号分子——细菌自诱导物-2(AI-2)在游动孢子卵菌中的产生。触发了哈维氏弧菌 AI-2 报告菌发光的 P. nicotianae、P. sojae 和 P. aphanidermatum 的 ZFF,表明 AI-2 存在于游动孢子细胞外产物中,并且卵菌和细菌之间存在跨界通讯的潜力。通过化学分析证实了游动孢子产生 AI-2。这些结果为卵菌的生理学和生态学提供了新的见解。