College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.
Lett Appl Microbiol. 2010 Feb;50(2):187-91. doi: 10.1111/j.1472-765X.2009.02773.x. Epub 2009 Nov 13.
To produce single cell protein (SCP) by using waste capsicum powder produced during capsanthin extraction as a substrate.
The extraction [CPM (capsicum powder medium)] from waste capsicum powder was used as culture medium to cultivate four yeast strains. The main composition of CPM was determined. The average concentration of total sugar, total nitrogen and phosphorous of CPM were 16.3, 3.7 g l(-1) and 785.4 mg l(-1), respectively. Four yeast strains were cultured in two CPMs, and the cell mass, protein content of cells and specific growth rate of cells were determined. Addition of corn steep liquor significantly increased the cell mass production. Presence of capsaicin in CPM did not show inhibition of cell growth of yeast tested.
CPM contained sufficient nutrients and could be used as a good medium to produce SCP. Candida utilis 1769 was chosen as the biomass producer because of its highest SCP formation (6.8 g l(-1)) and higher specific growth rate (0.12 h(-1)). The amino acid composition of its protein was well balanced.
Utilization of waste capsicum powder can reduce environmental pollution and increase protein supply for animal feed.
以辣椒红素提取过程中产生的废辣椒粉末为底物生产单细胞蛋白（SCP）。
利用废辣椒粉末提取的[CPM（辣椒粉末培养基）]作为培养基培养四种酵母菌株。确定了 CPM 的主要成分。CPM 的总糖、总氮和磷的平均浓度分别为 16.3、3.7 g/L 和 785.4 mg/L。在两种 CPM 中培养四种酵母菌株，测定细胞质量、细胞蛋白质含量和细胞比生长速率。添加玉米浆显著增加了细胞质量的产生。CPM 中的辣椒素对所测试的酵母细胞生长没有表现出抑制作用。
CPM 含有足够的营养物质，可以作为生产 SCP 的良好培养基。由于其最高的 SCP 形成量（6.8 g/L）和更高的比生长速率（0.12 h-1），选择产朊假丝酵母 1769 作为生物量生产菌。其蛋白质的氨基酸组成均衡。
利用废辣椒粉末可以减少环境污染，并为动物饲料提供更多的蛋白质。
