Department of Biochemistry, Osmania University, Hyderabad 500007, India.
Department of Biochemistry and Bioinformatics, GSS, GITAM, A P., Gandhinagar 530045, India.
Molecules. 2022 Sep 23;27(19):6265. doi: 10.3390/molecules27196265.
In the current study, soil samples were gathered from different places where petrol and diesel filling stations were located for isolation of photosynthetic bacteria under anaerobic conditions using the paraffin wax-overlay pour plate method with Biebl and Pfennig's medium. The three isolated strains were named SMR 001 (Mallapur), NR MPPR (Nacahram) and N Raju MPPR (Karolbagh). The morphologies of the bacteria were examined with a scanning electron microscope (SEM). The phylogenetic relationship between strains was examined by means of 16S rRNA gene sequence analysis using NCBI-BLAST search and a phylogenetic tree. The sequenced data for were deposited with the National Centre for Biotechnology Research (NCBI). The total amino acids produced by the isolated bacteria were determined by HPLC. A total of 14 amino acids and their derivatives were produced by the SMR 001 strain. Among these, carnosine was found in the highest concentration (8553.2 ng/mL), followed by isoleucine (1818.044 ng/mL) and anserine (109.5 ng/mL), while NR MPPR was found to produce 12 amino acids. Thirteen amino acids and their derivatives were found to be produced from N Raju MPPR, for which the concentration of carnosine (21601.056 ng/mL) was found to be the highest, followed by isoleucine (2032.6 ng/mL) and anserine (227.4 ng/mL). These microbes can be explored for the scaling up of the process, along with biohydrogen and single cell protein production.
在本研究中，从位于汽油和柴油加油站不同位置的土壤样本中，采用石蜡覆盖倾注平板法，用 Biebl 和 Pfennig 培养基在厌氧条件下分离出光合细菌。分离出的三株菌分别命名为 SMR 001（Mallapur）、NR MPPR（Nacahram）和 N Raju MPPR（Karolbagh）。使用扫描电子显微镜（SEM）检查细菌的形态。通过使用 NCBI-BLAST 搜索和系统发育树分析 16S rRNA 基因序列，检查菌株之间的系统发育关系。将 的测序数据提交给国家生物技术研究中心（NCBI）。通过 HPLC 测定分离菌产生的总氨基酸。SMR 001 菌株共产生 14 种氨基酸及其衍生物，其中肌肽含量最高（8553.2 ng/mL），其次是异亮氨酸（1818.044 ng/mL）和高丝氨酸（109.5 ng/mL），而 NR MPPR 则产生 12 种氨基酸。N Raju MPPR 产生 13 种氨基酸及其衍生物，其中肌肽含量最高（21601.056 ng/mL），其次是异亮氨酸（2032.6 ng/mL）和高丝氨酸（227.4 ng/mL）。这些微生物可以用于扩大生产过程，同时生产生物氢和单细胞蛋白。
