36个鲤科微卫星基因座在高体雅罗鱼（Phoxinus phoxinus (L.)）和赤眼鳟（Scardinius erythrophthalmus (L.)）中的跨物种扩增
Cross-species amplification of 36 cyprinid microsatellite loci in Phoxinus phoxinus (L.) and Scardinius erythrophthalmus (L.).
作者信息
Holmen Johannes, Vøllestad Leif A, Jakobsen Kjetill S, Primmer Craig R
机构信息
Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biology, University of Oslo, PO Box 1050 Blindern, N-0316 Oslo, Norway.
出版信息
BMC Res Notes. 2009 Dec 14;2:248. doi: 10.1186/1756-0500-2-248.
BACKGROUND
To conduct phylogeographic or population genetic studies, an adequate number of DNA markers for the focal species are required. Due to severe unavailability of genotype markers of any kind for the species Eurasian minnow (Phoxinus phoxinus L.) and rudd (Scardinius erythrophthalmus L.), we set out to attempt cross-amplification of a set of microsatellite loci from related species.
FINDINGS
We tested 36 cyprinid microsatellite loci for cross-species amplification in minnow and rudd. Fifteen species-locus combinations produced amplifications in minnow, seven being polymorphic, while 18 combinations amplified in rudd, nine of these being polymorphic.
CONCLUSIONS
The positive cross-species amplifications present potential contributions to the establishment of genetic marker sets for population genetics studies of the two focal species.
背景
为了进行系统地理学或种群遗传学研究，需要为目标物种提供足够数量的DNA标记。由于欧亚米诺鱼（Phoxinus phoxinus L.）和赤睛鱼（Scardinius erythrophthalmus L.）这两个物种的任何类型的基因型标记都严重缺乏，我们着手尝试从相关物种中对一组微卫星位点进行跨物种扩增。
研究结果
我们测试了36个鲤科微卫星位点在米诺鱼和赤睛鱼中的跨物种扩增情况。15个物种-位点组合在米诺鱼中产生了扩增，其中7个具有多态性，而18个组合在赤睛鱼中扩增，其中9个具有多态性。
结论
阳性跨物种扩增为为这两个目标物种的种群遗传学研究建立遗传标记集提供了潜在贡献。
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