Shimoda N, Knapik E W, Ziniti J, Sim C, Yamada E, Kaplan S, Jackson D, de Sauvage F, Jacob H, Fishman M C
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
Genomics. 1999 Jun 15;58(3):219-32. doi: 10.1006/geno.1999.5824.
The zebrafish is the first vertebrate organism used for large-scale genetic screens seeking genes critical to development. These screens have been quite successful, with more than 1800 recessive mutations discovered that speak to morphogenesis of the vertebrate embryo. The cloning of the mutant genes depends on a dense genetic map. The 2000 markers we present here, using microsatellite (CA) repeats, provides 1.2-cM average resolution. One centimorgan in zebrafish is about 0. 74 megabase, so, for many mutations, these markers are close enough to begin positional cloning by YAC walks.
斑马鱼是首个用于大规模基因筛选以寻找对发育至关重要基因的脊椎动物。这些筛选颇为成功，已发现1800多个隐性突变，这些突变揭示了脊椎动物胚胎的形态发生。突变基因的克隆依赖于密集的遗传图谱。我们在此展示的2000个使用微卫星（CA）重复序列的标记，平均分辨率为1.2厘摩。斑马鱼中的1厘摩约为0.74兆碱基，因此，对于许多突变而言，这些标记足够接近，可以通过酵母人工染色体步移开始进行定位克隆。
