Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy and Gas Hydrate, Chinese Academy of Sciences, Guangzhou 510640, China.
Bioresour Technol. 2010 Jul;101(13):4895-9. doi: 10.1016/j.biortech.2009.11.051. Epub 2009 Dec 8.
A two-step liquid hot water pretreatment (TSLHW) was developed with the objective of achieving complete saccharification of both hemicellulose and cellulose of Eucalyptus grandis, thereby avoiding the problems associated with the use of strong acid catalysts. The first step of the pretreatment was studied in the temperature range 180-200 degrees C, and the highest yield of total xylose achieved was 86.4% after 20 min at 180 degrees C. The second-step of the pretreatment was studied in the temperature range 180-240 degrees C and for lengths of time of 0-60 min. The conversion rate of glucan was more sensitive to temperature than time. The optimum reaction conditions for the second step of the pretreatment with minimal degradation of sugars were 200 degrees C for 20 min. the total sugar recovery from E. grandis with the optimized pretreatment and 72 h enzymatic digestion, reached 96.63%, which is superior to the recovery from a single-step pretreatment with hot water or dilute acid.
两段式液态热水预处理（TSLHW）旨在实现对巨桉木聚糖和纤维素的完全糖化，从而避免使用强酸催化剂带来的问题。预处理的第一步在 180-200°C 的温度范围内进行，在 180°C 下 20 分钟后总木糖的最高收率达到 86.4%。第二步预处理在 180-240°C 的温度范围内和 0-60 分钟的时间范围内进行。葡聚糖的转化率对温度比对时间更为敏感。第二步预处理的最佳反应条件为 200°C 20 分钟，在优化的预处理和 72 小时酶解后，从巨桉中回收的总糖达到 96.63%，优于单一热水或稀酸预处理的回收。
