Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
Oral Oncol. 2010 Feb;46(2):96-9. doi: 10.1016/j.oraloncology.2009.10.010. Epub 2009 Dec 9.
Cigarette smoke creates a field of injury in the epithelial lining of the entire respiratory tract causing an increased risk for the development of malignant lesions. It is conceivable, therefore, that early genetic alterations, can be detected in oral mucosa of heavy smokers mainly those affected by lung cancer. As aneuploidy was shown to be an early event in oral carcinogenesis, we aimed to investigate the prevalence of aneuploid cells (ACs) in samples obtained from apparently normal looking oral mucosa of heavy smokers affected by lung cancer (LC). Two brush samples were collected from the oral mucosa of 152 subjects; 31 heavy smokers with LC, 59 heavy smokers without LC and 62 never-smokers. The samples were simultaneously analyzed for morphology and fluorescent in situ hybridization (FISH) using chromosomes 2 and 8 centromeric probes. Over 2% ACs were found in 23% of heavy smokers with LC compared to 12% in heavy smokers without LC and 5% of the never-smokers group (P=0.015). A trend was also noticed when comparing the group of heavy smokers without LC with the never-smokers (P=0.198). We conclude that heavy smokers harbour detectable chromosomal numerical aberrations in oral epithelial cells of normal looking mucosa. These aberrations are more frequently found in heavy smokers affected by LC.
香烟烟雾会在整个呼吸道的上皮衬里造成损伤区域，从而增加恶性病变发展的风险。因此，可以想象，在重度吸烟者的口腔黏膜中，尤其是那些患有肺癌的患者中，可以检测到早期的遗传改变。由于非整倍体是口腔癌发生的早期事件，我们旨在研究在受肺癌（LC）影响的重度吸烟者的看似正常的口腔黏膜中获得的样本中异常细胞（ACs）的发生率。从 152 名受试者的口腔黏膜中采集了两个刷子样本；31 名患有 LC 的重度吸烟者，59 名没有 LC 的重度吸烟者和 62 名从不吸烟者。同时对样本进行形态学和荧光原位杂交（FISH）分析，使用染色体 2 和 8 着丝粒探针。与没有 LC 的重度吸烟者的 12%和从不吸烟者的 5%相比，在患有 LC 的重度吸烟者中发现了 23%的样本存在超过 2%的 AC（P=0.015）。当将没有 LC 的重度吸烟者组与从不吸烟者组进行比较时，也注意到了一种趋势（P=0.198）。我们得出结论，重度吸烟者的口腔上皮细胞中存在可检测到的染色体数量异常。这些异常在患有 LC 的重度吸烟者中更为常见。
