Expert group for Molecular and Cellular Biology, Belgian Nuclear Research Center SCK*CEN, 2400 Mol, Belgium.
J Microbiol Methods. 2010 Feb;80(2):148-54. doi: 10.1016/j.mimet.2009.11.012. Epub 2009 Dec 7.
Thanks to their photosynthetic and nutritive properties, cyanobacteria of the Arthrospira genus are of interest as food supplements, as efficient oxygen producing life support system organisms for manned space flight, and for the production of biofuels. Despite these potential valuable applications, full genome sequences and genetic information in general on Arthrospira remain scarce. This is mainly due to the difficulty to extract sufficient high molecular weight nucleic acids from these filamentous cyanobacteria. In this article, an efficient and reproducible DNA extraction procedure for cyanobacteria of the genus Arthrospira was developed. The method is based on the combination of a soft mechanical lysis with enzymatic disruption of the cell wall. The comparison with other extraction protocols clearly indicates that this optimised method allows the recovery of a larger amount of DNA. Furthermore, the extracted DNA presents a high molecular weight, a reduced degradation and an excellent overall quality. It can be directly used for molecular biology purposes such as PCR, and clone library construction.
感谢其光合作用和营养特性，节旋藻属的蓝细菌作为食物补充剂具有很大的吸引力，因为它可以作为载人航天高效产氧生命支持系统的生物体，并且还可以用于生产生物燃料。尽管具有这些潜在的有价值的应用，但是关于节旋藻的完整基因组序列和一般遗传信息仍然很少。这主要是由于从这些丝状蓝细菌中提取足够高分子质量的核酸很困难。在本文中，开发了一种用于节旋藻属蓝细菌的高效且可重复的 DNA 提取程序。该方法基于温和的机械裂解与细胞壁酶促破坏的结合。与其他提取方案的比较清楚地表明，这种优化的方法可以回收更多量的 DNA。此外，提取的 DNA 具有高分子质量，降解程度降低，并且整体质量优异。它可以直接用于分子生物学用途，如 PCR 和克隆文库构建。
