Viti C, Ventura S, Lotti F, Capolino E, Tomaselli L, Giovannetti L
Centro di Studio dei Microrganismi Autotrofi, CNR, Università di Firenze, Italy.
Res Microbiol. 1997 Sep-Oct;148(7):605-11. doi: 10.1016/S0923-2508(97)88084-9.
Arthrospira maxima and A. platensis are two species of cyanobacteria cultivated and sold as health food, animal feed and source of food additives and fine chemicals. The genotypic diversity of several strains attributed to these two species on the basis of morphological criteria was investigated using very sensitive total DNA restriction profile analysis. The restriction profiles were obtained after sodium dodecyl sulphate polyacrylamide gel electrophoresis and silver staining. The unweighted pair-group method using arithmetic averages applied to the matrix of Dice similarity coefficient values clustered the electropherograms of the strains in two well-separated genotypic groups. These clusters corresponded to those obtained with morphological criteria. The molecular approach used was also able to type the examined strains.
极大螺旋藻和钝顶螺旋藻是两种作为健康食品、动物饲料以及食品添加剂和精细化学品来源进行培育和销售的蓝细菌。基于形态学标准，利用非常灵敏的总DNA限制性图谱分析，研究了归属于这两个物种的多个菌株的基因型多样性。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和银染获得限制性图谱。采用算术平均值的非加权配对组方法应用于迪西相似系数值矩阵，将菌株的电泳图谱聚类为两个明显分开的基因型组。这些聚类与通过形态学标准获得的聚类相对应。所采用的分子方法也能够对检测的菌株进行分型。
