Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Nancy University, CNRS, UMR 7564, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, France.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Feb;75(2):610-6. doi: 10.1016/j.saa.2009.11.026. Epub 2009 Nov 18.
Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy is a useful method for monitoring biofilm in situ, non-destructively, in real time, and under fully hydrated conditions. In this work we focused on changes in Pseudomonas fluorescens ATR-FTIR fingerprint accompanying the very early stages of biofilm formation: initial bacterial adhesion and the very beginning of biofilm development in the presence of nutrients. To help interpreting variations in the ATR-FTIR fingerprint of sessile bacteria, ATR-FTIR spectra of planktonic bacteria in different growth phases were also examined, and the average surface coverage and spatial arrangement of bacteria on the ATR crystal were determined by epifluorescence microscopy. The proteins, nucleic acids and polysaccharides ATR-FTIR spectral data recorded during growth of sessile bacteria were shown to be linked to changes in the physiological state of the bacteria, possibly accompanied by extracellular polymeric substances production. This work clearly showed by spectroscopic method how bacteria change drastically their metabolism during the first hours of biofilm formation.
衰减全反射傅里叶变换红外（ATR-FTIR）光谱学是一种用于原位、非破坏性、实时监测水合条件下生物膜的有用方法。在这项工作中，我们专注于荧光假单胞菌 ATR-FTIR 指纹图谱随生物膜形成早期阶段的变化：营养物质存在时初始细菌附着和生物膜发展的初始阶段。为了帮助解释固定细菌的 ATR-FTIR 指纹图谱中的变化，还检查了不同生长阶段浮游细菌的 ATR-FTIR 光谱，并通过荧光显微镜确定了细菌在 ATR 晶体上的平均表面覆盖率和空间排列。在固定细菌生长过程中记录的蛋白质、核酸和多糖 ATR-FTIR 光谱数据与细菌生理状态的变化有关，可能伴随着细胞外聚合物的产生。这项工作通过光谱方法清楚地表明，在生物膜形成的最初几个小时内，细菌的新陈代谢发生了巨大变化。
