Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093-0412, USA.
Osteoarthritis Cartilage. 2010 Mar;18(3):464-71. doi: 10.1016/j.joca.2009.11.008. Epub 2009 Nov 23.
To determine the effects of acute injury and tribosupplementation by hyaluronan (HA) on synovial fluid (SF) modulation of cartilage shear during tibio-femoral articulation.
Human osteochondral blocks from the lateral femoral condyle (LFC) and tibial plateau (LTP) were apposed, compressed 13%, and subjected to sliding under video microscopy. Tests were conducted with equine SF from normal joints (NL-SF), SF from acutely injured joints (AI-SF), and AI-SF to which HA was added (AI-SF+HA). Local and overall shear strain (E(xz)) and the lateral displacement (Deltax) at which E(xz) reached 50% of peak values (Deltax(1/2)) were determined.
During articulation, LFC and LTP cartilage E(xz) increased with Deltax and peaked when surfaces slid, with peak E(xz) being maintained during sliding. With AI-SF as lubricant, surface and overall Deltax(1/2) were approximately 40% and approximately 20% higher, respectively, than values with NL-SF and AI-SF+HA as lubricant. Also, peak E(xz) was markedly higher with AI-SF as lubricant than with NL-SF as lubricant, both near the surface (approximately 80%) and overall (50-200%). Following HA supplementation to AI-SF, E(xz) was reduced from values with AI-SF alone by 30-50% near the surface and 20-30% overall. Magnitudes of surface and overall E(xz) were markedly (approximately 50 to 80%) higher in LTP cartilage than LFC cartilage for all lubricants.
Acute injury impairs SF function, elevating cartilage E(xz) markedly during tibio-femoral articulation; such elevated E(xz) may contribute to post-injury associated cartilage degeneration. Since HA partially restores the function of AI-SF, as indicated by E(xz), tribosupplements may be beneficial in modulating normal cartilage homeostasis.
确定急性损伤和透明质酸（HA）的摩擦补充对胫骨股骨关节中滑液（SF）调节软骨剪切的影响。
将来自外侧股骨髁（LFC）和胫骨平台（LTP）的人骨软骨块贴合，压缩 13%，并在视频显微镜下进行滑动。测试使用来自正常关节的马 SF（NL-SF）、来自急性损伤关节的 SF（AI-SF）和添加 HA 的 AI-SF（AI-SF+HA）进行。确定局部和整体剪切应变（E(xz))和达到峰值 E(xz) 50%的横向位移（Deltax(1/2))。
在关节运动过程中，LFC 和 LTP 软骨 E(xz)随 Deltax 增加而增加，并在表面滑动时达到峰值，在滑动过程中保持峰值 E(xz)。当使用 AI-SF 作为润滑剂时，表面和整体 Deltax(1/2)分别比使用 NL-SF 和 AI-SF+HA 作为润滑剂时高约 40%和 20%。此外，当使用 AI-SF 作为润滑剂时，与使用 NL-SF 作为润滑剂时相比，E(xz)的峰值明显更高，在表面附近（约 80%）和整体（50-200%）。在用 AI-SF 补充 HA 后，E(xz)在表面附近比单独使用 AI-SF 时降低了 30-50%，整体降低了 20-30%。对于所有润滑剂，LTP 软骨的表面和整体 E(xz)的幅度明显（约 50-80%）高于 LFC 软骨。
急性损伤会损害 SF 的功能，在胫骨股骨关节中显著提高软骨 E(xz)；这种升高的 E(xz)可能导致损伤后相关的软骨退化。由于 HA 部分恢复了 AI-SF 的功能，如 E(xz)所示，摩擦补充剂可能有益于调节正常软骨的动态平衡。
