Department of Life Science, College of Natural Sciences, Sangmyung University, Seoul 110-743, South Korea.
Mol Phylogenet Evol. 2010 Mar;54(3):1016-20. doi: 10.1016/j.ympev.2009.12.003. Epub 2009 Dec 21.
Class Gastropoda includes a large number of described species, many with extensively rearranged mitochondrial genomes. We sequenced the mitogenome of the rock shell, Thais clavigera (Gastropoda: Muricidae), an intertidal snail, using long PCR with primers designed on the basis of expressed sequence tags. The mitogenome of T. clavigera consists of 2 rRNAs, 22 tRNAs, and 13 protein-coding genes, but no control region. Structural comparisons revealed that the order Sorbeoconcha, including T. clavigera, have nearly identical mitochondrial gene patterns. However, they have an inversion between a tRNA(Phe)-tRNA(Glu) cluster that comprises 21 genes, but most of the remaining structure is similar to the putative mollusk ground pattern. These findings will provide a better insight into mitochondrial gene rearrangement over the course of gastropod evolution.
腹足纲包括大量已描述的物种，其中许多物种的线粒体基因组有广泛的重排。我们使用基于表达序列标签设计的引物，通过长 PCR 对潮间带蜗牛塔螺（腹足纲：宝螺科）的线粒体基因组进行了测序。T. clavigera 的线粒体基因组由 2 个 rRNA、22 个 tRNA 和 13 个蛋白编码基因组成，但没有控制区。结构比较表明，包括 T. clavigera 在内的索贝康恰（Sorbeoconcha）具有几乎相同的线粒体基因模式。然而，它们在包含 21 个基因的 tRNA(Phe)-tRNA(Glu)簇之间发生了倒位，但其余大部分结构与假定的软体动物基础模式相似。这些发现将为了解腹足类动物进化过程中的线粒体基因重排提供更好的认识。
