一种用于制备小体积缺氧溶液或液体培养基的真空涡旋技术，用于培养产甲烷菌或其他严格厌氧菌。
A vacuum-vortex technique for preparation of anoxic solutions or liquid culture media in small volumes for cultivating methanogens or other strict anaerobes.
机构信息
Department of Microbiology, University of Illinois, B 103 Chemical Life Sciences Laboratory, 601 S. Goodwin Ave., Urbana, IL 61801, USA.
出版信息
Anaerobe. 2010 Jun;16(3):216-9. doi: 10.1016/j.anaerobe.2009.11.005. Epub 2009 Dec 23.
Abstract
A highly efficient method is described for producing at room temperature anoxic solutions of 50 ml or less in test tubes or serum vials by combining negative pressure with strong vortexing so that the liquid-surface, gas exchange area is increased by orders of magnitude. Liquid media suitable for the cultivation of methanogens may be rendered anoxic after three short vacuum-vortex steps.
摘要
本文描述了一种在室温下在试管或血清小瓶中产生 50ml 或更少体积的缺氧溶液的高效方法，该方法通过将负压与强涡旋相结合，使液体表面、气体交换面积增加了几个数量级。适用于培养产甲烷菌的液体培养基可以通过三个短暂的真空-涡旋步骤来实现缺氧。
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