Centre for Environmental Sciences, Hasselt University, Agoralaan, Building D, 3590 Diepenbeek, Belgium.
J Plant Physiol. 2010 Mar 1;167(4):247-54. doi: 10.1016/j.jplph.2009.09.015. Epub 2009 Dec 11.
The leaf proteome of 3-week-old Arabidopsis thaliana seedlings exposed for 1 week to low, environmentally realistic Cd concentrations was investigated. The data indicated that at 1muMCd, A. thaliana plants adapted their metabolism to cope with the Cd exposure. As a result, only moderate protein changes were observed. However, at 10muMCd, severe stress was indicated by growth reduction and chlorosis of rosette leaves at the macroscopic level and by lipid peroxidation and enhanced peroxidase activity at the cellular level. Of the 730 reproducible proteins among all gels, 21 were statistically upregulated in response to Cd. These proteins can be functionally grouped into 5 classes: proteins involved in (1) oxidative stress response, (2) photosynthesis and energy production, (3) protein metabolism, (4) gene expression and finally, (5) proteins with various or unknown function. In order to provide greater insight into the mechanisms induced on Cd exposure, a working model is proposed.
对暴露于低浓度、环境现实 Cd 浓度下 1 周的 3 周龄拟南芥幼苗的叶片蛋白质组进行了研究。数据表明，在 1μMCd 下，拟南芥植物适应了其新陈代谢以应对 Cd 暴露。结果，仅观察到中等程度的蛋白质变化。然而，在 10μMCd 下，生长减少和莲座叶宏观水平的黄化以及细胞水平的脂质过氧化和增强的过氧化物酶活性表明存在严重的胁迫。在所有凝胶中可重复的 730 种蛋白质中，有 21 种因 Cd 而被统计地上调。这些蛋白质可以按功能分为 5 类：（1）参与氧化应激反应的蛋白质，（2）光合作用和能量产生，（3）蛋白质代谢，（4）基因表达，最后，（5）具有各种或未知功能的蛋白质。为了更深入地了解 Cd 暴露诱导的机制，提出了一个工作模型。
