Gallo Valentina, Zappettini Andrea, Villani Marco, Marmiroli Nelson, Marmiroli Marta
Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43123 Parma, Italy.
Institute of Materials for Electronics and Magnetism (IMEM), National Research Council (CNR), 43124 Parma, Italy.
Nanomaterials (Basel). 2021 Mar 1;11(3):615. doi: 10.3390/nano11030615.
In previous work, two independent Ac/Ds transposon insertional mutant lines, and were identified that showed a higher level of tolerance than the wild type (wt) line to cadmium sulfide quantum dots (CdS QDs). The tolerance response was characterized at physiological, genetic and transcriptomic levels. In this work, a comparative analysis was performed on protein extracts from plantlets of the two mutants and of wt, each treated with 80 mg L CdS QDs. A comparative protein analysis was performed by 2D-PAGE, and proteins were characterized by MALDI-TOF/TOF mass spectrometry. Of 250 proteins identified from all three lines, 98 showed significant changes in relative abundance between control and CdS QD-treated plantlets. The wt, , and control-treated pairs respectively showed 61, 31, and 31 proteins with differential expression. The two mutants had a different response to treatment in terms of type and quantity of up- and downregulated proteins. This difference became more striking when compared to wt. A network analysis of the proteins differentially expressed in and included several of those encoded by putative genes accommodating the transposons, which were responsible for regulation of some proteins identified in this study. These included nifu-like protein 3 (Nfu3), involved in chloroplast assembly, elongator complex 3 (Elo3), involved in transcriptional elongation, magnesium-chelate subunit-2 (Chli2), involved in chlorophyll biosynthesis, and protein phosphatase 2C (PP2C) which mediates abiotic stress response.
在之前的研究中，鉴定出了两个独立的Ac/Ds转座子插入突变体系，[体系名称未给出]，它们对硫化镉量子点（CdS QDs）的耐受性高于野生型（wt）品系。在生理、遗传和转录组水平上对耐受性反应进行了表征。在本研究中，对两个突变体和野生型幼苗的蛋白质提取物进行了比较分析，每个样本均用80 mg/L CdS QDs处理。通过二维聚丙烯酰胺凝胶电泳（2D-PAGE）进行了比较蛋白质分析，并用基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF）对蛋白质进行了表征。在从所有三个品系中鉴定出的250种蛋白质中，有98种在对照和CdS QDs处理的幼苗之间相对丰度有显著变化。野生型、[突变体名称未给出1]和[突变体名称未给出2]对照处理组分别有61、31和31种蛋白质差异表达。两个突变体在蛋白质上调和下调的类型和数量方面对处理有不同的反应。与野生型相比，这种差异更加显著。对[突变体名称未给出1]和[突变体名称未给出2]中差异表达的蛋白质进行的网络分析包括了一些由容纳转座子的推定基因编码的蛋白质，这些基因负责调控本研究中鉴定出的一些蛋白质。这些蛋白质包括参与叶绿体组装的类Nfu蛋白3（Nfu3）、参与转录延伸的延伸因子复合物3（Elo3）、参与叶绿素生物合成的镁螯合亚基2（Chli2）以及介导非生物胁迫反应的蛋白磷酸酶2C（PP2C）。
