State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Institute of Environmental Health & Ecological Security, Nanjing University, Nanjing, Jiangsu 210093, China.
J Hazard Mater. 2010 Apr 15;176(1-3):300-5. doi: 10.1016/j.jhazmat.2009.11.028. Epub 2009 Nov 13.
Reproductive toxicity of purified terephthalic acid (PTA) manufacturing wastewater on the male mice (Mus musculus) was investigated after 35-day intragastric perfusion treatment with the wastewater. Fluorescein diacetate and propidium iodide staining, and flow cytometry were used to assess the toxicity of PTA wastewater on spermatogenic cells. PTA wastewater induced significant variations in the relative percentages of immature haploid, diploid, tetraploid and S-phase spermatogonia. Percentage of viable spermatogenic cells was reduced from 93.1+/-2.3 in control group to 90.4+/-1.9 in the wastewater-treated group. Testicular histopathology revealed expansion of interstitial space and reduction in the number and size of Leydig cells induced by the wastewater, which was further certified by the decrease (10.6%) in relative testes weight and the increase (101.3%) in sperm shape abnormality in the wastewater-treated group. In this study, PTA wastewater was found to have reproductive toxicity on male mice, and public health problems may potentially arise from the discharge of the wastewater into the environment.
采用灌胃染毒方式，以 35 天为一个染毒周期，研究了精对苯二甲酸（PTA）生产废水对雄性昆明种小白鼠的生殖毒性。采用荧光素二乙酸酯（FDA）/碘化丙啶（PI）双染色和流式细胞术检测了 PTA 生产废水对雄性小白鼠生精细胞的毒性作用。结果显示，PTA 生产废水可引起生精细胞中未成熟的单倍体、二倍体、四倍体和 S 期细胞的比例显著变化。与对照组相比，生精细胞的存活率由 93.1%±2.3%降低至 90.4%±1.9%。睾丸组织病理学观察显示，废水可导致睾丸间质细胞间隙扩张，Leydig 细胞数量和体积减少，这一结果进一步被相对睾丸重量（减少 10.6%）和精子畸形率（增加 101.3%）的检测结果所证实。该研究表明，PTA 生产废水对雄性小白鼠具有生殖毒性，将该废水排放到环境中可能会对公共健康造成危害。
