Laboratoire de Biophysique Moléculaire, Université des Sciences et Technologies de Lille, Bâtiment C6, 59655 Villeneuve d'Ascq Cédex, France.
J Pharm Biomed Anal. 2010 Apr 6;51(5):1097-102. doi: 10.1016/j.jpba.2009.11.011. Epub 2009 Nov 13.
Fluorescence lifetimes of human serum albumin (HSA) tryptophan 214 residue were measured in solution at different pH (from 2 to 12). The results indicate that tryptophan emission occurs with three lifetimes at all pH. However, lifetimes and pre-exponential values are dependent on the pH and thus on the protein form. Three different protein populations have been differentiated: one population for pH 2 and 3 (extended form), the second one from pH 4 to 9 containing HSA migrating (F), normal (N) and basic (B) forms. Another type of population is obvious for pH higher than 9, characterizing the aged (A) form of HSA.
在不同 pH 值（2 至 12）下测量了人血清白蛋白（HSA）色氨酸 214 残基在溶液中的荧光寿命。结果表明，在所有 pH 值下，色氨酸发射都具有三种寿命。然而，寿命和预指数值取决于 pH 值，因此也取决于蛋白质形式。已经区分了三种不同的蛋白质群体：一种群体用于 pH 值为 2 和 3（扩展形式），另一种群体从 pH 值 4 到 9 包含迁移的 HSA（F）、正常（N）和碱性（B）形式。对于 pH 值高于 9 的另一种类型的群体是明显的，其特征是 HSA 的老化（A）形式。
