Department of Integrative Medical Biology, Section of Anatomy, Umeå University, SE-901 87 Umeå, Sweden.
J Plast Reconstr Aesthet Surg. 2010 Nov;63(11):1893-9. doi: 10.1016/j.bjps.2009.11.024. Epub 2009 Dec 14.
Peripheral nerve injuries are often associated with loss of nerve tissue and require autologous nerve grafts to provide a physical substrate for axonal growth. Biosynthetic neural conduits could be an alternative treatment strategy in such injuries. The present study investigates the long-term effects of a tubular fibrin conduit on neuronal regeneration, axonal sprouting and recovery of muscle weight following peripheral nerve injury and repair in adult rats. Sciatic axotomy was performed proximally in the thigh to create a 10-mm gap between the nerve stumps. The injury gap was bridged by using a 14-mm-long fibrin glue conduit, entubulating 2 mm of the nerve stump at each end. A reversed autologous nerve graft was used as a control. The regenerative response from sensory and motor neurones was evaluated following retrograde labelling with Fast Blue fluorescent tracer. In control experiments, at 16 weeks following peripheral nerve grafting, 5184 (±574 standard error of mean (SEM)) sensory dorsal root ganglion neurones and 1001 (±37 SEM) spinal motor neurones regenerated across the distal nerve-graft interface. The fibrin conduit promoted regeneration of 60% of sensory neurones and 52% of motor neurones when compared to the control group. The total number of myelinated axons in the distal nerve stump in the fibrin-conduit group reached 86% of the control and the weight of gastrocnemius and soleus muscles recovered to 82% and 89% of the controls, respectively. The present results suggest that a tubular fibrin conduit can be used to promote neuronal regeneration following peripheral nerve injury.
周围神经损伤常伴有神经组织丧失，需要自体神经移植为轴突生长提供物理基质。生物合成神经导管可能是此类损伤的一种替代治疗策略。本研究探讨了管状纤维蛋白导管对周围神经损伤和修复后成年大鼠神经元再生、轴突发芽和肌肉重量恢复的长期影响。在大腿近端进行坐骨神经切断术，在神经残端之间形成 10mm 的间隙。通过使用 14mm 长的纤维蛋白胶导管桥接损伤间隙，在每个神经残端的两端包埋 2mm 的神经来实现。将逆行自体神经移植作为对照。使用 Fast Blue 荧光示踪剂逆行标记评估感觉和运动神经元的再生反应。在对照实验中，在周围神经移植后 16 周，5184（±574 标准误差平均值（SEM））个感觉背根神经节神经元和 1001（±37 SEM）个脊髓运动神经元再生穿过远端神经移植界面。与对照组相比，纤维蛋白导管促进了 60%的感觉神经元和 52%的运动神经元的再生。纤维蛋白导管组远端神经残端的有髓轴突总数达到对照组的 86%，腓肠肌和比目鱼肌的重量分别恢复到对照组的 82%和 89%。本研究结果表明，管状纤维蛋白导管可用于促进周围神经损伤后的神经元再生。
