Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital Basel, Basel, Switzerland.
Department of Neuropathology, Institute of Pathology, University Hospital Basel, Basel, Switzerland.
Brain Behav. 2018 Jul;8(7):e01027. doi: 10.1002/brb3.1027. Epub 2018 Jun 19.
The aim was to evaluate the regenerative effect of epineural injection of rat ASCs (rASCs) in three different settings of acute and chronic compression in a rat sciatic nerve model.
Acute compression (60 s) with a vessel clamp over a distance of 1 mm (group 1) or 10 mm (group 2), as well as chronic compression with a permanent remaining, nonabsorbable polymeric clip over a distance of 1 mm (group 3) was performed. Depending on the group, either 5 × 10 rASCs or the same volume (25 μl) of culture medium (CM) was injected with a 30G needle in the epineurium at the time of compression. Outcome measures were functional gait evaluations, imaging analysis, histomorphometric analyses, and muscle weight.
The rats in group 2 had a better function than those with group 1 at one and especially at 2 weeks. After 4 weeks however, almost all rats were close to a normal function. There was a similar Muscle Weight Ratio (MWR) after 2 weeks in all groups, whereas after 4 weeks, the MWR in group 3 was lower compared with group 1 and 2. Histomorphometric analysis showed a better myelination in group 1 & 2 compared to group 3 after 4 weeks. ASCs have a beneficial effect on myelin thickness (G-Ratio).
We successfully evaluated the regenerative effect of epineural injection of rASCs in three different settings of acute and chronic compression. However, there were no significant differences in outcomes between the ASC-treated groups and control groups.
本研究旨在评估在大鼠坐骨神经模型中，三种不同急性和慢性压迫情况下，神经外膜注射大鼠脂肪间充质干细胞（rASCs）的再生效果。
采用血管夹在 1mm（第 1 组）或 10mm（第 2 组）的距离内进行急性压迫，以及采用永久性不可吸收聚合物夹在 1mm 的距离内进行慢性压迫。根据组别，在压迫时，用 30G 针将 5×10rASCs 或相同体积（25μl）的培养基（CM）注入神经外膜。评估指标包括功能步态评估、影像学分析、组织形态计量学分析和肌肉重量。
第 2 组大鼠在 1 周和特别是 2 周时的功能优于第 1 组。然而，4 周后，几乎所有大鼠的功能都接近正常。所有组在 2 周时的肌肉重量比（MWR）相似，而在 4 周时，第 3 组的 MWR 明显低于第 1 组和第 2 组。组织形态计量学分析显示，4 周后，第 1 组和第 2 组的髓鞘形成明显优于第 3 组。rASCs 对髓鞘厚度（G 比值）有有益的影响。
我们成功地评估了在三种不同的急性和慢性压迫情况下，神经外膜注射 rASCs 的再生效果。然而，在接受 ASC 治疗的组和对照组之间，结果没有显著差异。
